重组小鼠Hsp60蛋白(ab92364)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
描述
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产品名称
重组小鼠Hsp60蛋白
参阅全部 Hsp60 蛋白酶 -
生物活性
ATPase Activity Assay: Positive
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纯度
> 90 % SDS-PAGE.
>90% pure as determined by SDS-PAGE and Western blot analyses. This protein does not contain E. coli GroEL as demonstrated by western blot analysis. -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mouse
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab92364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.158% Tris HCl, 0.0292% EDTA, 0.87% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- 60 kDa chaperonin
- 60 kDa heat shock protein, mitochondrial
- CH60_HUMAN
see all -
功能
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
疾病相关
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
序列相似性
Belongs to the chaperonin (HSP60) family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
图片
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SDS-PAGE Analysis:
Lane 1: Molecular weight markers
Lane 2: ab92364 at 2.0 μg -
All lanes : Hsp60 monoclonal antibody at 1 µg/ml
Lane 1 : Molecular weight markers
Lane 2 : ab92364 at 0.1 µg
Lane 3 : E. coli GroEL Protein at 0.1 µg -
All lanes : GroEL monoclonal antibody at 1 µg/ml
Lane 1 : Molecular weight markers
Lane 2 : ab92364 at 0.1 µg
Lane 3 : E. coli GroEL Protein at 0.1 µg
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab92364 被引用在 1 文献中.
- Zhang H et al. Muscone Inhibits the Excessive Inflammatory Response in Myocardial Infarction by Targeting TREM-1. Evid Based Complement Alternat Med 2022:9112479 (2022). PubMed: 35591864