Recombinant小鼠Granzyme B protein (ab50114)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected BTI-TN-5B1-4 cells
  • 氨基酸序列
    • 种属Mouse

技术指标

Our Abpromise guarantee covers the use of ab50114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Determined by its ability to cleave a synthetic chromogentic Granzyme B substrate. The expected specific activity, when using the Ac-IEPD-pNA substrate at 25°C, is greater than 750 nM/min per µg of enzyme.
  • 应用

    SDS-PAGE

    Functional Studies

  • 内毒素水平< 0.100 Eu/µg
  • 纯度> 95 % SDS-PAGE.
    Greater than 98% by SDS-PAGE and HPLC analyses.
  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.

常规信息

  • 别名
    • C11
    • Cathepsin G like 1
    • Cathepsin G-like 1
    • CCPI
    • CGL 1
    • CGL1
    • CSP B
    • CSPB
    • CTLA-1
    • CTLA1
    • CTSGL1
    • Cytotoxic serine protease B
    • Cytotoxic T lymphocyte associated serine esterase 1
    • Cytotoxic T lymphocyte proteinase 2
    • Cytotoxic T-lymphocyte proteinase 2
    • Fragmentin 2
    • Fragmentin-2
    • GRAB_HUMAN
    • Granzyme 2
    • Granzyme B
    • Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1)
    • Granzyme-2
    • GranzymeB
    • GRB
    • Gzmb
    • Hlp
    • Human lymphocyte protein
    • Lymphocyte protease
    • Protease, serine, B
    • SECT
    • T cell serine protease 1-3E
    • T-cell serine protease 1-3E
    see all
  • 功能This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
  • 序列相似性Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • 细胞定位Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
  • Information by UniProt

Recombinant mouse Granzyme B protein (ab50114)参考文献

ab50114 has not yet been referenced specifically in any publications.

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