重组小鼠GAPDH蛋白(Active) (ab222428)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Mouse
    • 序列
      MGSSHHHHHHSSGLVPRGSHMGSMVKVGVNGFGRIGRLVTRAAICSGKVE IVAINDPFIDLNYMVYMFQYDSTHGKFNGTVKAENGKLVINGKPITIFQE RDPTNIKWGEAGAEYVVESTGVFTTMEKAGAHLKGGAKRVIISAPSADAP MFVMGVNHEKYDNSLKIVSNASCTTNCLAPLAKVIHDNFGIVEGLMTTVH AITATQKTVDGPSGKLWRDGRGAAQNIIPASTGAAKAVGKVIPELNGKLT GMAFRVPTPNVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTED QVVSCDFNSNSHSSTFDAGAGIALNDNFVKLISWYDNEYGYSNRVVDLMA YMASKE
    • 分子量
      38 kDa including tags
    • 氨基酸
      1 to 333
    • 标签
      His tag N-Terminus
    • 额外的序列信息
      NP_032110.

技术指标

Our Abpromise guarantee covers the use of ab222428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    Specific activity is > 40 units/mg, and is defined as the amount of enzyme that converts 1.0 µmole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37°C.

  • 应用

    Mass Spectrometry

    SDS-PAGE

    Functional Studies

  • 质谱法
    MALDI-TOF
  • 纯度
    >95% by SDS-PAGE .
    ab222428 was purified using conventional chromatography techniques.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 0.02% DTT, 20% Glycerol, PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • 38 kDa BFA-dependent ADP-ribosylation substrate
    • aging associated gene 9 protein
    • Aging-associated gene 9 protein
    • BARS-38
    • cb609
    • EC 1.2.1.12
    • Epididymis secretory sperm binding protein Li 162eP
    • G3P_HUMAN
    • G3PD
    • G3PDH
    • GAPD
    • GAPDH
    • Glyceraldehyde 3 phosphate dehydrogenase
    • Glyceraldehyde-3-phosphate dehydrogenase
    • HEL-S-162eP
    • KNC-NDS6
    • MGC102544
    • MGC102546
    • MGC103190
    • MGC103191
    • MGC105239
    • MGC127711
    • MGC88685
    • OCAS, p38 component
    • OCT1 coactivator in S phase, 38-KD component
    • peptidyl cysteine S nitrosylase GAPDH
    • Peptidyl-cysteine S-nitrosylase GAPDH
    • wu:fb33a10
    see all
  • 功能
    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • 通路
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • 序列相似性
    Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • 翻译后修饰
    S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • 细胞定位
    Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Information by UniProt

图片

  • 15% SDS-PAGE analysis of 3 µg ab222428.

文献

ab222428 has not yet been referenced specifically in any publications.

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