Recombinant小鼠FTO protein (ab108958)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ8BGW1
    • 种属Mouse
    • 分子量55 kDa including tags
    • 氨基酸2 to 502
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab108958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 内毒素水平< 1.000 Eu/µg
  • 纯度> 90 % SDS-PAGE.
    ab108958 is urified using Ni-NTA column and FPLC and 0.2µm filtered.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 55mM Tris HCl, 150mM Sodium chloride, pH 8.2

常规信息

  • 别名
    • AlkB homolog 9
    • ALKBH9
    • Alpha-ketoglutarate-dependent dioxygenase FTO
    • AW743446
    • Fat mass and obesity-associated protein
    • FATSO, MOUSE, HOMOLOG OF
    • Fto
    • FTO_HUMAN
    • GDFD
    • KIAA1752
    • mKIAA1752
    • Protein fatso
    see all
  • 功能Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.
  • 组织特异性Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.
  • 疾病相关Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GRDDCFED) [MIM:612938]. The disease consists of a severe children multiple congenital anomaly syndrome with death by the age of 3 years. All affected individuals had postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits, and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate were also observed.
  • 序列相似性Belongs to the fto family.
  • 结构域The 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases.
  • 细胞定位Nucleus.
  • Information by UniProt

Recombinant Mouse FTO protein (ab108958)参考文献

ab108958 has not yet been referenced specifically in any publications.

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