重组小鼠FGF21蛋白(Active) (ab216199)
Key features and details
- Expression system: Escherichia coli
- Purity: > 97% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, HPLC, SDS-PAGE
描述
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产品名称
重组小鼠FGF21蛋白(Active)
参阅全部 FGF21 蛋白酶 -
生物活性
ab216199 is fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2.0 × 103 IU/mg in the presence of 5 µg/ml of rMuKlotho-β and 10 μg/ml of heparin.
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纯度
> 97 % SDS-PAGE.
>97 % by HPLC. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
序列
AYPIPDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGAAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL LEDGYNVYQS EAHGLPLRLP QKDSPNQDAT SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY AS -
预测分子量
20 kDa -
氨基酸
29 to 210 -
额外的序列信息
A single non-glycosylated polypeptide chain containing 182 amino acids. This product is for the mature full length protein. The signal peptide is not included.
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技术指标
Our Abpromise guarantee covers the use of ab216199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
HPLC
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 100% PBS
Lyophilized from a 0.2 µm filtered concentrated solution in 3 × PBS.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Briefly centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml.
常规信息
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别名
- FGF 21
- FGF-21
- Fgf21
see all -
功能
Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. -
序列相似性
Belongs to the heparin-binding growth factors family. -
细胞定位
Secreted. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab216199 尚未被引用在任何文献中。