The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
内毒素水平< 0.100 Eu/µg
% SDS-PAGE. ab50235 purity is greater than 98% by SDS-PAGE and HPLC analyses.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
复溶Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use. Repeated freeze thaw cycles will result in loss of activity.
Basic fibroblast growth factor
Basic fibroblast growth factor bFGF
Fibroblast growth factor 2
Fibroblast growth factor 2 (basic)
Fibroblast growth factor, basic
Heparin binding growth factor 2 precursor
Heparin-binding growth factor 2
功能Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
组织特异性Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
序列相似性Belongs to the heparin-binding growth factors family.
翻译后修饰Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
细胞定位Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.