Recombinant小鼠Dnmt1 protein (ab91367)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus
  • 氨基酸序列
    • 种属Mouse
    • 氨基酸290 to 1620

技术指标

Our Abpromise guarantee covers the use of ab91367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity: 17 units/ul. Definition of specific activity: 1 unit is defined as the amount of the enzyme that transfer 1 pmole of methyl group to poly dI-dC substrate during 30 minutes at 37°C.
  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Liquid
  • 补充说明The Dnmt1 Reaction Buffer (5x) and S-adenosylmethionine (SAM) are NOT included. Customers will have to obtain these before starting their experiment. Reaction Conditions: Incubate in 1 x Dnmt1 Reaction Buffer (20mM Tris-HCl, pH7.4, 0.5 mM EDTA, 0.2 mM DTT, 5% glycerol) with 10µM S-adenosylmethionine (SAM) at 37°C.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 0.2M Sodium chloride, 10mM HEPES, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • ADCADN
    • AIM
    • CXXC finger protein 9
    • CXXC-type zinc finger protein 9
    • CXXC9
    • DNA (cytosine 5 ) methyltransferase 1
    • DNA (cytosine-5)-methyltransferase 1
    • DNA methyltransferase 1
    • DNA methyltransferase HsaI
    • DNA methyltransferase M.HsaI.
    • DNA MTase
    • DNA MTase HsaI
    • DNMT
    • DNMT 1
    • Dnmt1
    • DNMT1_HUMAN
    • Dnmt1o
    • FLJ16293
    • HSN1E
    • M.HsaI
    • MCMT
    • Met1
    • MGC104992
    • mMmul
    • MommeD2
    see all
  • 功能Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
  • 组织特异性Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
  • 序列相似性Belongs to the C5-methyltransferase family.
    Contains 2 BAH domains.
    Contains 1 CXXC-type zinc finger.
  • 结构域The N-terminal part is required for homodimerization and acts as a regulatory domain.
  • 翻译后修饰Sumoylated; sumoylation increases activity.
  • 细胞定位Nucleus.
  • Information by UniProt

Recombinant mouse Dnmt1 protein 图像

  • SDS-PAGE showing ab91367 at approximately 140kDa.

Recombinant mouse Dnmt1 protein (ab91367)参考文献

ab91367 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab91367.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"