The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生物活性Inhibits adipogenesis of 3T3L1 cells. Induces Hes1 in 3T3L1 cells. Interacts with Notch receptors.
内毒素水平< 0.100 Eu/µg
% SDS-PAGE. ab108549 is 0.2µm filtered.
补充说明Working aliquots are stable for up to 3 months when stored at -20°C.
Inhibits adipogenesis of 3T3L1 cells. Induces Hes1 in 3T3L1 cells. Interacts with Notch receptors.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Delta (Drosophila) like 1
Delta like 1
Delta like 1 (Drosophila)
Delta like 1 protein
Delta like protein 1
Delta like protein 1 precursor
Delta, Drosophila, homolog of 1
Delta-like protein 1
Drosophila Delta homolog 1
功能Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
组织特异性Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
Adipogenesis inhibition of 3T3L1 cells.
3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin.
Adipogenesis was initiated by adding 1µM Dexamethasone, 0.5mM IBMX, 10µg/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5µg/ml mDLL1-Fc (ab108549) and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol.
Functional Studies - DLL1 protein (ab108549)
Induction of Hes-1 with the treatment of recombinant mouse DLL1-Fc (ab108549).
A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5mg/ml of mouse DLL1-Fc (ab108549) as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
Lane 1: ab108549, 0 min
Lane 2: ab108549, 10 min
Lane 3: ab108549, 30 min
Lane 4: ab108549, 1 hr
Lane 5: ab108549, 2 hr
Lane 6: ab108549, 4 hr
Lane 7: ab108549, 8 hr
Lane 8: ab108549, 24 hr
Recombinant Mouse DLL1 protein (ab108549)参考文献
has not yet been referenced specifically in any publications.