Recombinant小鼠DLL1 protein (ab108549)

概述

描述

  • 性质Recombinant
  • 来源HEK 293 cells
  • 氨基酸序列
    • AccessionQ61483
    • 种属Mouse
    • 分子量100 kDa including tags
    • 氨基酸1 to 545

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技术指标

Our Abpromise guarantee covers the use of ab108549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Inhibits adipogenesis of 3T3L1 cells. Induces Hes1 in 3T3L1 cells. Interacts with Notch receptors.
  • 应用

    Functional Studies

    SDS-PAGE

  • 内毒素水平< 0.100 Eu/µg
  • 纯度> 90 % SDS-PAGE.
    ab108549 is 0.2µm filtered.
  • 形式Liquid
  • 补充说明Working aliquots are stable for up to 3 months when stored at -20°C. Inhibits adipogenesis of 3T3L1 cells. Induces Hes1 in 3T3L1 cells. Interacts with Notch receptors.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS

常规信息

  • 别名
    • Delta
    • Delta (Drosophila) like 1
    • Delta like 1
    • Delta like 1 (Drosophila)
    • Delta like 1 protein
    • Delta like protein 1
    • Delta like protein 1 precursor
    • Delta, Drosophila, homolog of 1
    • Delta-like protein 1
    • Delta1
    • DL1
    • DLL 1
    • DLL1
    • DLL1_HUMAN
    • Drosophila Delta homolog 1
    • H-Delta-1
    • OTTHUMP00000017690
    see all
  • 功能Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
  • 组织特异性Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
  • 序列相似性Contains 1 DSL domain.
    Contains 8 EGF-like domains.
  • 翻译后修饰Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
  • 细胞定位Membrane.
  • Information by UniProt

Recombinant Mouse DLL1 protein 图像

  • Adipogenesis inhibition. Macroscopic pictures.
  • Adipogenesis inhibition of 3T3L1 cells.
    3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1µM Dexamethasone, 0.5mM IBMX, 10µg/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5µg/ml mDLL1-Fc (ab108549) and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol.
  • Induction of Hes-1 with the treatment of recombinant mouse DLL1-Fc (ab108549).
    A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5mg/ml of mouse DLL1-Fc (ab108549) as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.

    Lane 1: ab108549, 0 min
    Lane 2: ab108549, 10 min
    Lane 3: ab108549, 30 min
    Lane 4: ab108549, 1 hr
    Lane 5: ab108549, 2 hr
    Lane 6: ab108549, 4 hr
    Lane 7: ab108549, 8 hr
    Lane 8: ab108549, 24 hr

Recombinant Mouse DLL1 protein (ab108549)参考文献

ab108549 has not yet been referenced specifically in any publications.

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