The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
2uM ab111143 generated a 9-fold ATPase activation of 2uM Hsp90 (His-tagged Hsp90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees celcius (this is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).
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Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.