Recombinant小鼠AHA1 protein (ab111143)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ8BK64
    • 种属Mouse
    • 分子量38 kDa
    • 氨基酸1 to 338
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab111143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性2uM ab111143 generated a 9-fold ATPase activation of 2uM Hsp90 (His-tagged Hsp90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees celcius (this is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).
  • 应用

    Functional Studies

    Western blot

  • 纯度> 90 % SDS-PAGE.

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    pH: 7.20
    Constituents: 0.88% Sodium chloride, 0.002% DTT, 50% Glycerol, 0.378% Tris HCl

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Activator of 90 kDa heat shock protein ATPase homolog 1
    • Activator of heat shock 90kDa protein ATPase homolog 1
    • AHA 1
    • AHA1
    • AHSA 1
    • Ahsa1
    • AHSA1_HUMAN
    • C14orf3
    • HSPC322
    • p38
    see all
  • 功能Cochaperone that stimulates HSP90 ATPase activity (By similarity). May affect a step in the endoplasmic reticulum to Golgi trafficking.
  • 组织特异性Expressed in numerous tissues, including brain, heart, skeletal muscle and kidney and, at lower levels, liver and placenta.
  • 序列相似性Belongs to the AHA1 family.
  • 细胞定位Cytoplasm > cytosol. Endoplasmic reticulum. May transiently interact with the endoplasmic reticulum.
  • Information by UniProt

Recombinant mouse AHA1 protein (ab111143)参考文献

ab111143 has not yet been referenced specifically in any publications.

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