重组Measles virus Priorix,Schwarz strain,nucleocapsid蛋白(ab74559)
Key features and details
- Expression system: Saccharomyces cerevisiae
- Purity: > 95% SDS-PAGE
- Suitable for: WB, ELISA, SDS-PAGE
描述
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产品名称
重组Measles virus Priorix,Schwarz strain,nucleocapsid蛋白 -
纯度
> 95 % SDS-PAGE.
ab74559 is purified by ultracentifugation. -
表达系统
Saccharomyces cerevisiae -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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序列
MATLLRSLALFKRNKDKPPITSGSGGAIRGIKHIIIVPIPGDSSITTRSR LLDRLVRLIGNPDVSGPKLTGALIGILSLFVESPGQLIQRITDDPDVSIR LLEVVQSDQSQSGLTFASRGTNMEDEADQYFSHDDPISSDQSRFGWFGNK EISDIEVQDPEGFNMILGTILAQIWVLLAKAVTAPDTAADSELRRWIKYT QQRRVVGEFRLERKWLDVVRNRIAEDLSLRRFMVALILDIKRTPGNKPRI AEMICDIDTYIVEAGLASFILTIKFGIETMYPALGLHEFAGELSTLESLM NLYQQMGETAPYMVILENSIQNKFSAGSYPLLWSYAMGVGVELENSMGGL NFGRSYFDPAYFRLGQEMVRRSAGKVSSTLASELGITAEDARLVSEIAMH TTEDKISRAVGPRQAQVSFLHGDQSENELPRLGGKEDRRVKQSRGEARES YRETGPSRASDARAAHLPTGTPLDIDTATESSQDPQDSRRSADALLRLQA MAGISEEQGSDTDTPIVYNDRNLLD -
预测分子量
58 kDa -
氨基酸
1 to 525
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技术指标
Our Abpromise guarantee covers the use of ab74559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
ELISA
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Constituent: PBS
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复溶Lyophilised, reconstitute with 120µl deionised water and add glycerol to 50%
常规信息
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别名
- N
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相关性
Priorix is a new investigational measles, mumps and rubella (MMR) vaccine containing the Schwarz measles strain, the RA 27/3 rubella strain and a new mumps strain RIT 4385.
图片
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All lanes : Recombinant Measles virus Priorix, Schwarz strain, nucleocapsid protein (ab74559)
Lanes 1-2 : S. cerevisiae cell lysates without measles nucleoprotein (negative controls)
Lane 3 : S. cerevisiae cell lysate with recombinantly expressed measles nucleoprotein
Lane 4 : CsCl purified measles Nucleoprotein from S. cerevisiae
Predicted band size: 60 kDa -
All lanes : Recombinant Measles virus Priorix, Schwarz strain, nucleocapsid protein (ab74559)
Lanes 1-2 : S. cerevisiae cell lysates without measles nucleoprotein (negative controls)
Lane 3 : S. cerevisiae cell lysate with recombinantly expressed measles nucleoprotein
Lane 4 : CsCl purified measles Nucleoprotein from S. cerevisiae
Predicted band size: 60 kDaSDS-PAGE and WB of yeast lysates and purified Measles Nucleoprotein (ab74559) on a 12% polyacrylamide gel. Immunoblot using measles positive human serum.
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All lanes : Recombinant Measles virus Priorix, Schwarz strain, nucleocapsid protein (ab74559)
Lanes 1-2 : S. cerevisiae cell lysates without measles nucleoprotein (negative controls)
Lane 3 : S. cerevisiae cell lysate with recombinantly expressed measles nucleoprotein
Lane 4 : CsCl purified measles Nucleoprotein from S. cerevisiae
Predicted band size: 60 kDaSDS-PAGE and WB of yeast lysates and purified Measles Nucleoprotein (ab74559) on a 12% polyacrylamide gel.
Immunoblot using Mab against measles nucleoprotein
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SDS-PAGE showing ab74559 at approximately 60kDa. (2 μg/lane).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab74559 被引用在 3 文献中.
- Böröcz K et al. Application of a fast and cost-effective 'three-in-one' MMR ELISA as a tool for surveying anti-MMR humoral immunity: the Hungarian experience. Epidemiol Infect 148:e17 (2020). PubMed: 32014073
- Hong J et al. Correlation between the results of two analytical methods for measuring measles virus neutralizing antibodies in source plasma and therapeutic immunoglobulin products. Biologicals 59:20-28 (2019). PubMed: 30992162
- Richardson SI et al. IgG3 enhances neutralization potency and Fc effector function of an HIV V2-specific broadly neutralizing antibody. PLoS Pathog 15:e1008064 (2019). PubMed: 31841557