Recombinant Listeriolysin (LLO) protein (ab68198)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 氨基酸26 to 529

技术指标

Our Abpromise guarantee covers the use of ab68198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Activity: ab68198 has a hemolytic activity 1.25x106 HU/mg. 2mM DTT is used to reactivate the toxin.
  • 应用

    SDS-PAGE

  • 纯度> 90 % SDS-PAGE.
    Purity: Greater than 90.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE
  • 形式Liquid
  • 补充说明


    This protein can be used for: Cytosolic delivery of molecules, peptides, oligonucleodides and plasmid DNA.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 5% Glycerol, 0.5M Sodium chloride, 50mM Sodium dihydrogen phosphate, 2.7mM Potassium chloride, 1mM DTT, 1mM EDTA

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • hly
    • hlyA
    • lisA
    • LLO
    • Thiol activated cytolysin
    see all
  • 相关性Listeria monocyogenes is a faculative intracellular Gram-positive food borne bacterium, increasingly recognized as being responsible for severe infections in both humans and animals. Ingestion of contaminated food causes an infection called Listeriosis, which affects especially immunocomprimised patients, new borns and pregnant women. It is characterized by severe syndromes such as encephalitis, meningoencephalitis, septicemia and abortion. Listeriolysin O (LLO) is a single polypeptide protein secreted by the Gram-positive bacterium Listeria monocytogenes. LLO belongs to the group of cholesterol-binding sulfhydrylactivated toxins, the lytic activity of which is enhanced by reducing agents and is suppressed by exposure to oxygen or cholesterol. LLO hemolytic activity is maximum at pH 5.5 and rapidly decreases with the increase of the pH.

Recombinant Listeriolysin (LLO) protein (ab68198)参考文献

ab68198 has not yet been referenced specifically in any publications.

Product Wall

The unit of activity mentioned on the datasheet of ab68198, Listeriolysin full length protein, HU/mg (hemolytic units) is defined in the attached paper,

High-level expression of the Listeria monocytogenes listeriolysin O in Escherichia coli an...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"