重组JCV Polyomavirus Major Capsid VP1蛋白(ab74569)
Key features and details
- Expression system: Saccharomyces cerevisiae
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, SDS-PAGE
描述
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产品名称
重组JCV Polyomavirus Major Capsid VP1蛋白
参阅全部 JCV Polyomavirus Major Capsid VP1 蛋白酶 -
生物活性
JCV VP1 protein was purified and lyophilized assembled into virus like particles (VLPs). It is tested for hemagglutination activity and analysed using electron microscopy.
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纯度
> 95 % SDS-PAGE.
Purified by ultracentifugation. -
表达系统
Saccharomyces cerevisiae -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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序列
MAPTKRKGERKDPVQVPKLLIRGGVEVLEVKTGVDSITEVECFLTPEMGD PDEHLRGFSKSISISDTFESDSPNKDMLPCYSVARIPLPNLNEDLTCGNI LMWEAVTLKTEVLGVTTLMNVHSNGQATHDNGAGKPVQGTSFHFFSVGGE ALELQGVVFNYRTKYPDGTIFPKNATVQSQVMNTEHKAYLDKNKAYPVEC WVPDPTRNENTRYFGTLTGGENVPPVLHITNTATTVLLDEFGVGPLCKGD NLYLSAVDVCGMFTNRSGSQQWRGLSRYFKVQLRKRRVKNPYPISFLLTD LINRRTPKVDGQPMYGMDAQIEEVRVFEGTEELPGDPDMMRYVDRYGQLQ TKML -
预测分子量
40 kDa -
实际分子量
41 kDa -
氨基酸
1 to 354
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab74569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
SDS-PAGE
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形式
Lyophilized -
补充说明
JCV VP1 protein was purified and lyophilized assembled into virus like particles (VLPs). It is tested for hemagglutination activity and analysed using electron microscopy.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C.
Constituent: PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with deionized H2O. After reconstitution store at 4°C. With the addition of glycerol store at -20°C.
常规信息
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别名
- Capsid protein VP1
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相关性
The human polyomavirus JC virus (JCV) infects greater than 80% of the human population. The JC virus is a small (38-40 nm in diameter) double stranded, circular DNA virus covered by an icosahedral capsid. Infection with JCV is asymptomatic and it occurs in early childhood. After the primary infection, the virus remains in latent state in the kidney, until it's reactivation under immunosuppressive conditions to result in Progressive Multifocal Leukoencephalopathy (PML), a fatal demyelinating disease. 70% of all HIV-1- infected patients will exhibit neurological disorders and between 5 and 8% of all HIV-1-infected patients will develop PML. Similar to other polyomaviruses, JCV can cause tumors when intracerebrally inoculated at high titers into developing rodent. Several reports suggest the association of viruses, especially of the polyomavirus family with different types of human brain tumors. Tumorigenecity of JCV is most likely induced by the viral early gene product T-antigen. T-antigen has the capacity to interact with several tumor suppressor proteins, most notably p53, and functionally inactivate these proteins. -
细胞定位
Virion. Nucleus
图片
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Recombinant JCV Polyomavirus Major Capsid VP1 protein (ab74569)
Predicted band size: 40 kDaWestern blot showing ab74569 (Lane 1)
MW ladder (Lane 2)
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SDS-PAGE showing ab74569 (Lane 1)
Lane 2 reprents the molecular weight ladder. -
SDS-PAGE showing ab74569 (4µg/lane).
Lane 1 reprents the molecular weight ladder. From the bottom: 14.4, 18.4, 25.0, 35.0, 45.0, 66.2 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (3)
ab74569 被引用在 3 文献中.
- López-Moncada F et al. Nucleolar Expression and Chromosomal Associations in Robertsonian Spermatocytes of Mus musculus domesticus. Genes (Basel) 10:N/A (2019). PubMed: 30736350
- Tan CS et al. Brief Report: Decreased JC Virus-Specific Antibody-Dependent Cellular Cytotoxicity in HIV-Seropositive PML Survivors. J Acquir Immune Defic Syndr 82:220-224 (2019). PubMed: 31513076
- Saccardo P et al. Effect of the DnaK chaperone on the conformational quality of JCV VP1 virus-like particles produced in Escherichia coli. Biotechnol Prog 30:744-8 (2014). PubMed: 24574306