Recombinant人ZIP Kinase protein (ab63192)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionO43293
    • 种属Human
    • 氨基酸1 to 454
    • 标签GST tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab63192 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity: 37 nmol/min/mg.
  • 应用

    SDS-PAGE

    Functional Studies

  • 形式Liquid
  • 补充说明

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • DAP kinase 3
    • DAP like kinase
    • DAP-like kinase
    • Dapk 3
    • DAPK3
    • DAPK3_HUMAN
    • Death associated kinase 3
    • Death associated protein kinase 3
    • Death-associated protein kinase 3
    • Dlk
    • EC 2.7.11.1
    • FLJ36473
    • MYPT1 kinase
    • ZIP
    • ZIP kinase
    • ZIP kinase isoform
    • ZIP-kinase
    • ZIPK
    • zipper-interacting protein kinase
    see all
  • 功能Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus.
    Autophosphorylated. Phosphorylated by ROCK1.
  • 细胞定位Nucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase.
  • Information by UniProt

Recombinant human ZIP Kinase protein 图像

  • Sample Kinase Activity Plot.
  • ab63192 on SDS-PAGE. Molecular Weight: ~79 kDa.

Recombinant human ZIP Kinase protein (ab63192)参考文献

ab63192 has not yet been referenced specifically in any publications.

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