概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP04628
    • 种属Human
    • 序列ANSSGRWWGI VNVASSTNLL TDSKSLQLVL EPSLQLLSRK QRRLIRQNPG ILHSVSGGLQ SAVRECKWQF RNRRWNCPTA PGPHLFGKIV NRGCRETAFI FAITSAGVTH SVARSCSEGS IESCTCDYRR RGPGGPDWHW GGCSDNIDFG RLFGREFVDS GEKGRDLRFL MNLHNNEAGR TTVFSEMRQE CKCHGMSGSC TVRTCWMRLP TLRAVGDVLR DRFDGASRVL YGNRGSNRAS RAELLRLEPE DPAHKPPSPH DLVYFEKSPN FCTYSGRLGT AGTAGRACNS SSPALDGCEL LCCGRGHRTR TQRVTERCNC TFHWCCHVSC RNCTHTRVLH ECL
    • 分子量38 kDa
    • 氨基酸28 to 370
    • 额外的序列信息This protein is a non-glycosylated full length mature protein that does not contain the signal peptide from aa 1 - 27.

技术指标

Our Abpromise guarantee covers the use of ab84080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

    SDS-PAGE

  • 内毒素水平< 0.100 Eu/µg
  • 纯度> 95 % SDS-PAGE.
    Purity is greater than 98% by SDS-PAGE gel and HPLC analyses.
  • 形式Lyophilised
  • 补充说明Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstitute in water to a concentration of 0.1 – 1 mg/ml. ab84080 reconstituted is stable for at least 6 months when stored in working aliquots with a carrier protein such as 0.1% BSA at -20oC. Avoid repeated freeze/thaw cycles.

常规信息

  • 别名
    • BMND16
    • INT1
    • OI15
    • oncogene Int1
    • Proto oncogene protein Wnt 1
    • Proto-oncogene Int-1 homolog
    • Proto-oncogene Wnt-1
    • Wingless type MMTV integration site family member 1
    • wingless-type MMTV integration site family, member 1 (oncogene INT1)
    • Wnt 1
    • wnt1
    • WNT1_HUMAN
    see all
  • 功能Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters.
  • 序列相似性Belongs to the Wnt family.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

Recombinant human Wnt1 protein (ab84080)参考文献

This product has been referenced in:
  • Xie W  et al. Sox2 modulates Lef-1 expression during airway submucosal gland development. Am J Physiol Lung Cell Mol Physiol 306:L645-60 (2014). Mouse . Read more (PubMed: 24487391) »
  • Cai WY  et al. The Wnt-ß-catenin pathway represses let-7 microRNA expression through transactivation of Lin28 to augment breast cancer stem cell expansion. J Cell Sci 126:2877-89 (2013). Read more (PubMed: 23613467) »

See all 8 Publications for this product

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We have validated ab109822 for use with the biotinylated ab84243 and will guarantee these antibodies for use as an sELISA pair. For the positive control, I would recommend using the full length protein, such as ab...

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Thank you for your enquiry regarding ab84080. For ab84080, the initial reconstitution should be performed in water to a concentration of 0.1-1.0mg/ml. So, the maximum reconstitution concentration is 1mg/ml (1000ug/ml). I hope this information he...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"