Recombinant人WNK1 protein (ab105196)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionQ9H4A3
    • 种属Human
    • 分子量67 kDa including tags
    • 氨基酸181 to 507

技术指标

Our Abpromise guarantee covers the use of ab105196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The Specific activity of ab105196 was determined to be 30 nmol/min/mg.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    Purity was determined to be >95% by densitometry.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Erythrocyte 65 kDa protein
    • HSAN2
    • HSN2
    • hWNK1
    • KDP
    • KIAA0344
    • Kinase deficient protein
    • MGC163339
    • MGC163341
    • p65
    • PRKWNK1
    • Prostate derived sterile 20 like kinase
    • Protein kinase lysine deficient 1
    • Protein kinase lysine-deficient 1
    • Protein kinase with no lysine 1
    • PSK
    • Serine/threonine protein kinase WNK1
    • Serine/threonine-protein kinase WNK1
    • With no K
    • WNK lysine deficient protein kinase 1
    • WNK1
    • WNK1_HUMAN
    see all
  • 功能Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
  • 组织特异性Widely expressed, with highest levels observed in the testis, heart, kidney and skeletal muscle. Isoform 3 is kidney-specific and specifically expressed in the distal convoluted tubule (DCT) and connecting tubule (CNT) of the nephron.
  • 疾病相关Pseudohypoaldosteronism 2C
    Neuropathy, hereditary sensory and autonomic, 2A
  • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰O-glycosylated.
    Ubiquitinated in vitro by the BCR(KLHL3) complex and in vivo by a BCR(KLHL2) complex, leading to proteasomal degradation.
  • 细胞定位Cytoplasm.
  • Information by UniProt

Recombinant human WNK1 protein 图像

  • The Specific activity of ab105196 was determined to be 30 nmol/min/mg.
  • SDS-PAGE showing ab105196 at approximately 67kDa.

Recombinant human WNK1 protein (ab105196)参考文献

ab105196 has not yet been referenced specifically in any publications.

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