Recombinant人VRK1 protein (ab125555)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionQ99986
    • 种属Human
    • 分子量71 kDa including tags
    • 氨基酸1 to 396

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技术指标

Our Abpromise guarantee covers the use of ab125555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The specific activity of ab125555 was determined to be 2 nmol/min/mg.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度> 95 % Densitometry.
    Purity was determined to be >95% by densitometry.
  • 形式Liquid
  • 补充说明

    ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • MGC117401
    • MGC138280
    • MGC142070
    • PCH1
    • PCH1A
    • Serine/threonine protein kinase VRK1
    • Serine/threonine-protein kinase VRK1
    • Vaccinia related kinase 1
    • Vaccinia virus B1R related kinase 1
    • Vaccinia-related kinase 1
    • VRK1
    • VRK1_HUMAN
    see all
  • 功能Serine/threonine kinase that phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2.
  • 组织特异性Widely expressed. Highly expressed in fetal liver, testis and thymus.
  • 疾病相关Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1) [MIM:607596]; also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy (SMA).
  • 序列相似性Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53.
  • 细胞定位Nucleus. Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis.
  • Information by UniProt

Recombinant human VRK1 protein 图像

  • SDS-PAGE analysis of ab125555.
  • Sample Kinase Assay showing the Specific Activity of ab125555 to be 2 nmol/min/mg.

Recombinant human VRK1 protein (ab125555)参考文献

ab125555 has not yet been referenced specifically in any publications.

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