Recombinant人VEGFB protein (ab87052)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA
    • 氨基酸22 to 207

技术指标

Our Abpromise guarantee covers the use of ab87052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is < 1ng/ml.
    Activity Assay:
    1. Cell line: NIH-3T3 (Mus musculus/Mouse embryonic fibroblast)
    2. Maintenance Condition: RPMI 1640 containing 10% FBS
    3. Assay medium: serum free RPMI 1640
    4. Cell density: 2 x 104 cells/well (96 well plate, final volume 100ul)
    5. Incubation time : 24 hr (after sample treatment)
    6. Concentration range: 10pg/ml - 10ug/ml
    7. Detection method : BrdU assay
  • 应用

    Functional Studies

    SDS-PAGE

  • 内毒素水平< 1.000 Eu/µg
  • 质谱法
    MALDI-TOF
  • 纯度> 90 % SDS-PAGE.
    ab87052 was purified using conventional chromatography techniques
  • 形式Liquid
  • 补充说明Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method)
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Vascular endothelial growth factor B
    • VEGF B
    • VEGF related factor
    • VEGF-B
    • VEGF-related factor
    • Vegfb
    • VEGFB_HUMAN
    • VEGFL
    • VRF
    see all
  • 功能Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
  • 组织特异性Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.
  • 序列相似性Belongs to the PDGF/VEGF growth factor family.
  • 翻译后修饰VEGF-B186 is O-glycosylated.
  • 细胞定位Secreted. Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.
  • Information by UniProt

Recombinant human VEGFB protein 图像

Recombinant human VEGFB protein (ab87052)参考文献

ab87052 has not yet been referenced specifically in any publications.

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