Recombinant人VEGFA protein (ab55566)



  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 氨基酸5 to 169
    • 标签His tag N-Terminus
    • 额外的序列信息This recombinant VEGF protein comprises a 165 amino acid fragment (5-169) corresponding to the VEGF 165 isoform and is expressed in E. coli with an aminoterminal hexahistidine tag.


Our Abpromise guarantee covers the use of ab55566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用


    Western blot

  • 纯度> 95 % SDS-PAGE.

  • 形式Liquid
  • Concentration information loading...


  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 20mM Tris, pH 8


  • 别名
    • MGC70609
    • MVCD1
    • vascular endothelial growth factor
    • Vascular endothelial growth factor A
    • Vascular permeability factor
    • Vegf
    • VEGF A
    • VEGF-A
    • VEGF120
    • Vegfa
    • VPF
    see all
  • 功能Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • 组织特异性Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • 疾病相关Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 序列相似性Belongs to the PDGF/VEGF growth factor family.
  • 细胞定位Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt

Recombinant Human VEGFA protein 图像

  • ab55566 on SDS-PAGE

  • developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 90 seconds

Recombinant Human VEGFA protein (ab55566)参考文献

This product has been referenced in:
  • Mannell HK  et al. ARNO regulates VEGF-dependent tissue responses by stabilizing endothelial VEGFR-2 surface expression. Cardiovasc Res 93:111-9 (2012). Read more (PubMed: 22002459) »

See 1 Publication for this product

Product Wall

I am sorry my previous email was unclear.

The phosphorylation status of ab42614 is no known. This is not something that is determined during the production process.

I hope this information is helpful.

Thank you for your reply.

Actually it is fine to centrifuge agarose beads. Typically for IP I have centrifuged agarose beads at 500xg for 3-5 min at 4 degrees C.

Regarding your questions 1 and 2, those notes on the datasheet for ab4...

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Thank you for your enquiry.

I think I have found a product that will suit your needs:

This is anti-GST antibody covalently linked to agarose beads. Using this product ...

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I'm happy to help! Please contact me again if you need anything further.

Thank you for your enquiry.

VEGF Receptor 2 protein (Tagged) (ab42614) contains an N-terminal GST tag.

I hope this is helpful. Please contact me again if you have any further questions.

I am very pleased to hear you would like to accept our offer and test ab55566 inFunctional Studies.This code will give you 1 free protein before the expiration date. To redeem this offer, please submit an Abreview for Functional Studiesand include...

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Thank you very much for your interest in ab55566.

To our knowledge, ab55566 has not been tested in functional assays. Therefore, I can offer a discount off a future purchase if you buy ab55566 now, test it in a functional assayand submit fee...

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