重组人VEGFA蛋白(Active) (ab155740)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人VEGFA蛋白(Active)
参阅全部 VEGFA 蛋白酶 -
生物活性
The bio-activity was determined by dose-dependent stimulation of the proliferation of HUVEC cells.
The ED50 <5ng/ml, corresponding to a specific activity of >2x105 Unit/mg.
ab155740 at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF R2, His Tag with a linear range of 10-156 ng/mL.
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纯度
> 98 % SDS-PAGE.
>95% as determined by SEC-MALS. Purified by ion exchnage chromatography. -
内毒素水平
< 1.000 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPS CVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHN KCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQ LELNERTCRCDKPRR -
预测分子量
19 kDa -
氨基酸
27 to 191
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab155740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: PBS
Normally Mannitol or Trehalose are added as protectants before lyophilisation.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with sterile deionized water to a concentration of 200 µg/ml.
常规信息
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别名
- Folliculostellate cell-derived growth factor
- Glioma-derived endothelial cell mitogen
- MGC70609
see all -
功能
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. -
组织特异性
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. -
疾病相关
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
序列相似性
Belongs to the PDGF/VEGF growth factor family. -
细胞定位
Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. - Information by UniProt
图片
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Avastin (Bevacizumab) captured on CM5 chip via ant-human IgG Fc antibodies surface, can bind ab155740 with an affinity constant of 0.776 nM as determined in a SPR assay (Biacore T200).
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ab155740 on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.
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A SEC-HPLC analysis showing 95% of ab155740 present as active homodimers.
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Immobilized ab155740 at 2 µg/mL (100 µL/well) can bind pre-mixed increasing concentrations of Bevacizumab and 0.5 µg/mL (100 µL/well) Biotinylated Human VEGF R2 protein with a half maximal inhibitory concentration (IC50) of 0.70 µg/mL.
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Immobilized ab155740 at 2 µg/mL (100 µL/well) can bind Biotinylated Human VEGF R2 protein with a linear range of 10-156 ng/mL.
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Immobilized ab155740 at 0.01 µg/mL (100 µL/well) can bind Human VEGF R1 protein with a linear range of 4-31 ng/mL.
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Inhibition assay shows that the proliferation effect of ab155740 is inhibited by increasing concentration of anti-VEGF mAb (Avastin). The concentration of VEGF165 used is 20 ng/ml. The ED50 is 0.065-0.229 µg/mL.
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ab155740 stimulates proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is 4.216-9.281 ng/ml.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab155740 被引用在 1 文献中.
- Crawford DK et al. ELX-02 Generates Protein via Premature Stop Codon Read-Through without Inducing Native Stop Codon Read-Through Proteins. J Pharmacol Exp Ther 374:264-272 (2020). PubMed: 32376628