Recombinant人VEGF 121B protein (ab86935)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MGSSHHHHHH SSGLVPRGSH MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR
    • 氨基酸207 to 327

技术指标

Our Abpromise guarantee covers the use of ab86935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The ED50 for this effect is < 4.2 ng/ml. Measured in a cell proliferation assay using NIH-3T3 cell.
  • 应用

    Functional Studies

    SDS-PAGE

  • 内毒素水平< 1.000 Eu/µg
  • 质谱法
    MALDI-TOF
  • 纯度> 95 % SDS-PAGE.
    purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • MVCD1
    • Vascular endothelial growth factor A
    • Vascular permeability factor
    • VEGF
    • VEGF A
    • VPF
    see all
  • 相关性VEGFA is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF. VEGF 121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates.
  • 细胞定位Secreted

Recombinant human VEGF 121B protein 图像

  • 15% SDS Page analysis of 3ug ab86935
  • NIH/3T3 cell proliferation was determined by BrdU incorporation method at 405 nm. This test was performed duplicate.N.C. : negative control [only 0.1% FBS]
    P.C. : positive control [10% FBS]

Recombinant human VEGF 121B protein (ab86935)参考文献

ab86935 has not yet been referenced specifically in any publications.

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