产品名称 Recombinant人Urm1 protein 参阅全部 Urm1 蛋白酶
蛋白长度 Full length protein 描述
来源 Escherichia coli
序列 MGSSHHHHHHSSGLVPRGSHMAAPLSVEVEFGGGAELLFDGIKKHRVTLP GQEEPWDIRNLLIWIKKNLLKERPELFIQGDSVRPGILVLINDADWELLG ELDYQLQDQDSVLFISTLHGG
分子量 14 kDa including tags
氨基酸 1 to 101
标签 His tag N-Terminus 技术指标
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
纯度 > 95
% SDS-PAGE. ab105598 was purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
Concentration information loading... 制备和贮存
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0
Chromosome 9 open reading frame 74
功能 Acts as a sulfur carrier required for 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Serves as sulfur donor in tRNA 2-thiolation reaction by thiocarboxylated (-COSH) at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5)S(2)U. May also act as an ubiquitin-like protein that is covalently conjugated to other proteins; the relevance of such function is however unclear in vivo.
通路 tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.
序列相似性 Belongs to the URM1 family.
翻译后修饰 C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.
Information by UniProt
Recombinant Human Urm1 protein 图像
Recombinant Human Urm1 protein (ab105598)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"