Recombinant人Ubiquitin (mutated K6 + K11 + K27 + K29 + K33 + K63) protein (Chemical Free) (ab80796)

概述

  • 产品名称
    Recombinant人Ubiquitin (mutated K6 + K11 + K27 + K29 + K33 + K63) protein (Chemical Free)
    参阅全部 Ubiquitin 蛋白酶
  • 蛋白长度
    Full length protein

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 分子量
      9 kDa

技术指标

Our Abpromise guarantee covers the use of ab80796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    This ubiquitin mutant contains only a single lysine, K48, with all other lysines mutated to arginine. This mutation renders ubiquitin able to form poly-ubiquitin chains with other ubiquitin molecules exclusively via the K48 lysine.
  • 应用

    Conjugation

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.

  • 形式
    Lyophilised
  • 补充说明

    This ubiquitin mutant contains only a single lysine, K48, with all other lysines mutated to arginine. This mutation renders ubiquitin able to form poly-ubiquitin chains with other ubiquitin molecules exclusively via the K48 lysine.

    Typical concentrations for non rate-limiting support of in vitro conjugationreactions range from 200 μM-1 mM depending on experimental conditions.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    None

  • 复溶
    1 mg of lyophilized powder. Soluble and stable in aqueous buffers up to 10 mg/ml.

常规信息

  • 别名
    • Epididymis secretory protein Li 50
    • FLJ25987
    • HEL S 50
    • MGC8385
    • Polyubiquitin B
    • RPS 27A
    • RPS27A
    • UBA 52
    • UBA 80
    • UBA52
    • UBA80
    • UBB
    • UBB_HUMAN
    • UBC
    • UBCEP 1
    • UBCEP 2
    • UBCEP1
    • UBCEP2
    • Ubiquitin
    • Ubiquitin B
    see all
  • 相关性
    Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
  • 细胞定位
    Cell Membrane, Cytoplasmic and Nuclear

文献

ab80796 has not yet been referenced specifically in any publications.

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