Recombinant人Ubiquitin (mutated K48) protein (Chemical Free) (ab80740)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP62988
    • 种属Human
    • 分子量9 kDa
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab80740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Mutation of lysine 48 to arginine renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 48 linkages with other Ub molecules. ab80740 can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins (monoubiquitination). Ideal for the reduction in poly-Ub chain length/conjugation rates and for the determination of poly-Ub chains specificity.
  • 应用

    Conjugation

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Lyophilised
  • 补充说明

    Mutation of lysine 48 to arginine renders ubiquitin (Ub) unable to form poly-Ub chains via lysine 48 linkages with other Ub molecules. Ub K48R can form an E1-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins (monoubiquitination). Ideal for the reduction in poly-Ub chain length/conjugation rates and determining if poly-Ub chains are K48 linked.

    Typical concentrations for non rate-limiting support of in vitro conjugation reactions range from 200 μM-1 mM depending on experimental conditions.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    None

  • 复溶Soluble and stable in aqueous buffers up to 10 mg/ml. Store at -20°C after solubilization in desired buffer.

常规信息

  • 别名
    • FLJ25987
    • MGC8385
    • Polyubiquitin B
    • RPS 27A
    • RPS27A
    • UBA 52
    • UBA 80
    • UBA52
    • UBA80
    • UBB
    • UBB_HUMAN
    • UBC
    • UBCEP 1
    • UBCEP 2
    • UBCEP1
    • UBCEP2
    • Ubiquitin
    • Ubiquitin B
    see all
  • 相关性Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
  • 细胞定位Cell Membrane, Cytoplasmic and Nuclear

Recombinant Human Ubiquitin (mutated K48) protein (Chemical Free) (ab80740)参考文献

ab80740 has not yet been referenced specifically in any publications.

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