Recombinant人Ubiquitin 7-amido-4-methylcoumarin protein (Chemical Free) (ab81665)

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概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
      P0CG47
    • 种属
      Human
    • 分子量
      9 kDa
    • 额外的序列信息
      C-terminus derivatization of ubiquitin with 7-amido-4-methylcoumarin (AMC).

技术指标

Our Abpromise guarantee covers the use of ab81665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

  • 形式
    Lyophilised
  • 补充说明

    ab81665 is a fluorogenic substrate for ubiquitin hydrolases. Ubiquitin-AMC is an exquisitely sensitive substrate for UCH-L3 (Km = 0.039 µM) and for the Isopeptidase-T (Km = 0.17 - 1.4 µM). Ub-AMC is useful for studying ubiquitin hydrolases when detection sensitivity or continuous monitoring of activity is essential.

     

    Substrate concentrations for assay range from 0.1-1 μM. Typical enzyme concentrations for UCH-L3 are 10-100 pM and for Isopeptidase-T are 10-100 nM. Release of AMC fluorescence by UCH enzymes can be monitored using Ex380 nm and Em460 nm wavelengths, respectively.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 100% DMSO

常规信息

  • 别名
    • Ub-AMC
    • Ubiquitin-AMC
  • 相关性
    Ubiquitin 7-amido-4-methylcoumarin (Ub-AMC) is a sensitive fluorescence-based substrate of deubiquitinating enzymes. It is useful for monitoring deubiquitinating enzymes’ activity or screening inhibitors of deubiquitinating enzymes. The concentration of Ub-AMC used in deubiquitinating reactions is usually 0.1-1 µM. The released AMC fluorescence can be monitored using a fluorometer or plate reader with excitation and emission wavelengths at 360 nm and 460 nm, respectively.
  • 细胞定位
    Cytoplasmic and Nuclear

Recombinant Human Ubiquitin 7-amido-4-methylcoumarin protein (Chemical Free) (ab81665)参考文献

ab81665 has not yet been referenced specifically in any publications.

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