重组人Ube2N / Ubc13蛋白(ab95900)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
描述
-
产品名称
重组人Ube2N / Ubc13蛋白 -
纯度
> 80 % SDS-PAGE. -
表达系统
Escherichia coli -
Accession
-
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
预测分子量
18 kDa including tags -
氨基酸
2 to 152 -
标签
His tag N-Terminus
-
相关产品
-
Related Products
技术指标
Our Abpromise guarantee covers the use of ab95900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
SDS-PAGE
-
形式
Liquid -
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on Dry Ice. Store at -80°C.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
常规信息
-
别名
- Bendless like ubiquitin conjugating enzyme
- Bendless-like ubiquitin-conjugating enzyme
- BLU
see all -
功能
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the ubiquitin-conjugating enzyme family. -
翻译后修饰
Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab95900 尚未被引用在任何文献中。