Recombinant人UBE2D3 protein (ab59314)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列The sequence (minus the tag) is: MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDS PYQGGVFFLTIHFPTDY PFKPPKVAFTTRIYHPNINSNGSICLDILRS QWSPALTISKVLLSICSLLCDPNPDDPLV PEIARIYKTDREKYNRIAR EWTQKYAM

技术指标

Our Abpromise guarantee covers the use of ab59314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 50mM Sodium chloride, 50mM HEPES, 1mM DTT, pH 7.6

常规信息

  • 别名
    • E2(17)KB3
    • MGC43926
    • MGC5416
    • PRO2116
    • UB2D3_HUMAN
    • UBC 4/5
    • UBC4/5
    • UBC4/5 homolog yeast
    • UBC4/5, S. cerevisiae, homolog of
    • UBCH 5C
    • UBCH5C
    • Ube2d3
    • Ubiquitin carrier protein
    • Ubiquitin carrier protein D3
    • Ubiquitin conjugating enzyme E2 17 kDa 3
    • Ubiquitin conjugating enzyme E2 D3
    • Ubiquitin conjugating enzyme E2D 3
    • Ubiquitin conjugating enzyme E2D 3 (homologous to yeast UBC4/5)
    • Ubiquitin conjugating enzyme E2D 3 (UBC4/5 homolog yeast)
    • Ubiquitin protein ligase D3
    • Ubiquitin-conjugating enzyme E2 D3
    • Ubiquitin-conjugating enzyme E2(17)KB 3
    • Ubiquitin-conjugating enzyme E2-17 kDa 3
    • Ubiquitin-protein ligase D3
    see all
  • 功能Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair. Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML-NB) formation in the nucleus. In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53. Supports NRDP1-mediated ubiquitination and degradation of ERBB3 and of BRUCE which triggers apoptosis. In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes. In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the ubiquitin-conjugating enzyme family.
  • 细胞定位Cell membrane. Endosome membrane.
  • Information by UniProt

Recombinant Human UBE2D3 protein (ab59314)参考文献

ab59314 has not yet been referenced specifically in any publications.

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