Recombinant人Ube2B protein (ab73536)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MHHHHHHAMGQLRSMSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQW NAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGS ICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKRE YEKRVSAIVEQSWNDS
    • 额外的序列信息Ubiquitin Conjugating Enzyme E2B Human Recombinant produced in E.coli is a 19 kDa protein containing 166 amino acids.

技术指标

Our Abpromise guarantee covers the use of ab73536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    ab73536 is purified by proprietary chromatographic techniques. Purity greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • 形式Lyophilised
  • 补充说明Lyophilized Ube2B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 1mM DTT, 1X PBS, pH 7.5

  • 复溶Reconstitute the lyophilized Ube2B in sterile water at not less than 100µg/ml, which can then be further diluted to other aqueous solutions

常规信息

  • 别名
    • E2 17kDa
    • E2 protein
    • hHR6B
    • HR6B
    • RAD6 homolog B
    • RAD6B
    • UBC2
    • UBE2B
    • UBE2B_HUMAN
    • Ubiquitin carrier protein B
    • Ubiquitin conjugating enzyme E2 B
    • Ubiquitin conjugating enzyme E2B
    • Ubiquitin conjugating enzyme E2B (RAD6 homolog)
    • Ubiquitin protein ligase B
    • Ubiquitin-conjugating enzyme E2 17 kDa
    • Ubiquitin-conjugating enzyme E2 B
    • Ubiquitin-conjugating enzyme E2-17 kDa
    • Ubiquitin-protein ligase B
    see all
  • 功能Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the ubiquitin-conjugating enzyme family.
  • 细胞定位Cell membrane. Nucleus. In peripheral neurons, expressed both at the plasma membrane and in nuclei.
  • Information by UniProt

Recombinant Human Ube2B protein (ab73536)参考文献

ab73536 has not yet been referenced specifically in any publications.

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