Recombinant人TXNRD1 (mutated C498) protein (ab82727)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 分子量55 kDa including tags
    • 氨基酸1 to 498
    • 修饰mutated C498
    • 额外的序列信息There is an additional 3 amino acids-GSH at N-terminal. The cysteine at aa498 is mutated to selenocysteine.

技术指标

Our Abpromise guarantee covers the use of ab82727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Lyophilised
  • 补充说明

    The mammalian thioredoxin reductases (TrxRs) are a family of selenocysteine containing pyridine nucleotide-disulfide oxido-reductases. All the mammalian TrxRs

    are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with

    a C-terminal extension containing a catalytically active penultimate selenocysteine (SeCys) residue in the conserved sequence(-Gly-Cys-SeCys-Gly).

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 20mM HEPES, 1mM EDTA, pH 7

  • 复溶Reconstitute in 20mM HEPES, pH 7.0, Packaging size : 0.5 mg

常规信息

  • 别名
    • cytoplasmic
    • Gene associated with retinoic and IFN-induced mortality 12 protein
    • Gene associated with retinoic and interferon-induced mortality 12 protein
    • Gene associated with retinoid IFN induced mortality 12 protein
    • GRIM 12
    • GRIM-12
    • GRIM12
    • KDRF
    • KM 102 derived reductase like factor
    • KM-102-derived reductase-like factor
    • MGC9145
    • Oxidoreductase
    • Thioredoxin reductase 1
    • Thioredoxin reductase 1 cytoplasmic
    • Thioredoxin reductase GRIM 12
    • Thioredoxin reductase TR1
    • TR
    • TR 1
    • TR1
    • TRXR 1
    • TRXR1
    • TRXR1_HUMAN
    • TXNR
    • TXNRD 1
    • Txnrd1
    see all
  • 功能Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
  • 组织特异性Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.
  • 序列相似性Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
    Contains 1 glutaredoxin domain.
  • 结构域The N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays.
  • 翻译后修饰The N-terminus of isoform 5 is blocked.
    ISGylated.
  • 细胞定位Cytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt

Recombinant Human TXNRD1 (mutated C498) protein 图像

  • ab82727 at 1µg on SDS-PAGE.

Recombinant Human TXNRD1 (mutated C498) protein (ab82727)参考文献

ab82727 has not yet been referenced specifically in any publications.

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