概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • 种属
      Human
    • 分子量
      27 kDa

技术指标

Our Abpromise guarantee covers the use of ab9968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

    SDS-PAGE

  • 内毒素水平
    < 0.100 Eu/µg
  • 形式
    Lyophilised
  • 补充说明
    The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10ng/ml.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶
    Centrifuge the vial prior to opening. Reconstitute in 10mM Sodium Phosphate, pH7.5 to a concentration of 1mg/ml. The solution can be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for future use

常规信息

  • 别名
    • APO 3 ligand
    • APO 3L
    • APO3 ligand
    • APO3/DR3 ligand
    • APO3L
    • DR3LG
    • MGC129581
    • MGC20669
    • secreted form
    • TNF-related weak inducer of apoptosis
    • TNF12_HUMAN
    • TNFSF 12
    • Tnfsf12
    • TNFSF12 protein
    • Tumor necrosis factor (ligand) superfamily member 12
    • Tumor necrosis factor ligand superfamily member 12
    • Tumor necrosis factor superfamily member 12
    • TWEAK
    • UNQ181/PRO207
    see all
  • 功能
    Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines.
  • 组织特异性
    Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.
  • 序列相似性
    Belongs to the tumor necrosis factor family.
  • 翻译后修饰
    The soluble form derives from the membrane form by proteolytic processing.
  • 细胞定位
    Secreted and Cell membrane.
  • Information by UniProt

Recombinant human TWEAK protein (ab9968)参考文献

This product has been referenced in:
  • Qi X  et al. Lipopolysaccharide Upregulated Intestinal Epithelial Cell Expression of Fn14 and Activation of Fn14 Signaling Amplify Intestinal TLR4-Mediated Inflammation. Front Cell Infect Microbiol 7:315 (2017). Functional Studies ; Pig . Read more (PubMed: 28744451) »

See 1 Publication for this product

Product Wall

Thank you for your response. Our lab has confirmed that ab9968 does not contain any tags. The amino acid sequence for this protein is as follows:   MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG ...

Read More

Thank you for your question. I have been in touch with my contact at the lab and I was told that ab9968 is the soluble recombinant TWEAK protein. I hope this is helpful, but please let me know if you have further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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