重组人TrkC蛋白(Fc Chimera) (ab85599)

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概述

描述

  • 性质
    Recombinant
  • 来源
    HEK 293 cells
  • 氨基酸序列
    • Accession
      Q16288
    • 种属
      Human
    • 序列
      Theoretical Sequence: CPANCVCSKTEINCRRPDDGNLFPLLEGQDSGNSNGNANINITDISRN ITSIHIENWRSLHTLNAVDMELYTGLQKLTIKNSGLRSIQPRAFAKNPHL RYINLSSNRLTTLSWQLFQTLSLRELQLEQNFFNCSCDIRWMQLWQEQGE AKLNSQNLYCINADGSQLPLFRMNISQCDLPEISVSHVNLTVREGDNAVI TCNGSGSPLPDVDWIVTGLQSINTHQTNLNWTNVHAINLTLVNVTSEDNG FTLTCIAENVVGMSNASVALTVYYPPRVVSLEEPELRLEHCIEFVVRGNP PPTLHWLHNGQPLRESKIIHVEYYQEGEISEGCLLFNKPTHYNNGNYTLI AKNPLGTANQTINGHFLKEPFPESTDNFILFDEVSPTPPITVTHKPEEDG SSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISR TPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSV LTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSR DELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFF LYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
    • 氨基酸
      1 to 428
    • 额外的序列信息
      Fused with the Fc region of Human IgG1 at the C-terminus.

技术指标

Our Abpromise guarantee covers the use of ab85599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.

  • 形式
    Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin, PBS

  • 复溶
    Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

常规信息

  • 别名
    • EC 2.7.10.1
    • ETS related protein neurotrophic receptor tyrosine kinase fusion
    • ETS related protein neurotrophic receptor tyrosine kinase fusion protein
    • ETV6 NTRK3 fusion
    • GP145 TrkC
    • gp145(trkC)
    • GP145-TrkC
    • GP145TrkC
    • Neurotrophic tyrosine kinase receptor type 3
    • Neurotrophin 3 receptor
    • NT 3 growth factor receptor
    • NT 3 growth factor receptor precursor
    • NT 3 receptor
    • NT-3 growth factor receptor
    • Ntrk3
    • NTRK3_HUMAN
    • OTTHUMP00000192915
    • TRK C
    • Trk-C
    • TRKC
    • TrkC tyrosine kinase
    • Tyrosine kinase receptor C
    see all
  • 功能
    Receptor for neurotrophin-3 (NT-3). This is a tyrosine-protein kinase receptor. Known substrates for the trk receptors are SHC1, PI-3 kinase, and PLCG1. The different isoforms do not have identical signaling properties.
  • 组织特异性
    Widely expressed but mainly in nervous tissue. Isoform B is expressed at higher levels in adult brain than in fetal brain.
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Ligand-mediated auto-phosphorylation.
  • 细胞定位
    Membrane.
  • Information by UniProt

图片

  • SDS-PAGE - TrkC – Fc Chimera (ab85599)
    SDS-PAGE - TrkC – Fc Chimera (ab85599)
    1D SDS-PAGE of ab85599 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1: ab85599
    Lane 2: ab85599 treated with PNGase F to remove potential N-linked glycans
    Lane 3: ab85599 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
    5 mg of protein was loaded per lane. Gel was stained with Deep Purple™.

    Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Subsequent tightening of bands after treatment with glycosidase cocktail suggests the presence of O-linked glycans.

  • Functional Studies - TrkC – Fc Chimera (ab85599)
    Functional Studies - TrkC – Fc Chimera (ab85599)
    Densitometry of protein isoforms visualised by 2-DE.
    The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
    The triangle indicates the theoretical MW and pI of the protein.

文献

ab85599 has not yet been referenced specifically in any publications.

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