概述
- 产品名称Recombinant人TrkC protein (Fc Chimera)
参阅全部 TrkC 蛋白酶 - 蛋白长度Full length protein
描述
- 性质Recombinant
- 来源HEK 293 cells
- 氨基酸序列
- AccessionQ16288
- 种属Human
- 序列Theoretical Sequence: CPANCVCSKTEINCRRPDDGNLFPLLEGQDSGNSNGNANINITDISRN ITSIHIENWRSLHTLNAVDMELYTGLQKLTIKNSGLRSIQPRAFAKNPHL RYINLSSNRLTTLSWQLFQTLSLRELQLEQNFFNCSCDIRWMQLWQEQGE AKLNSQNLYCINADGSQLPLFRMNISQCDLPEISVSHVNLTVREGDNAVI TCNGSGSPLPDVDWIVTGLQSINTHQTNLNWTNVHAINLTLVNVTSEDNG FTLTCIAENVVGMSNASVALTVYYPPRVVSLEEPELRLEHCIEFVVRGNP PPTLHWLHNGQPLRESKIIHVEYYQEGEISEGCLLFNKPTHYNNGNYTLI AKNPLGTANQTINGHFLKEPFPESTDNFILFDEVSPTPPITVTHKPEEDG SSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISR TPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSV LTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSR DELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFF LYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
- 氨基酸1 to 428
- 额外的序列信息Fused with the Fc region of Human IgG1 at the C-terminus.
相关产品
- Related Products
技术指标
Our Abpromise guarantee covers the use of ab85599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 应用
Functional Studies
SDS-PAGE
- 纯度> 95
% SDS-PAGE.
- 形式Lyophilised
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Concentration information loading...
制备和贮存
- 稳定性和存储
Shipped at 4°C. Store at +4°C.
Preservative: None
Constituents: 10% Trehalose, 1% Human serum albumin, PBS - 复溶Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
常规信息
-
别名
- EC 2.7.10.1
- ETS related protein neurotrophic receptor tyrosine kinase fusion
- ETS related protein neurotrophic receptor tyrosine kinase fusion protein
see all - 功能Receptor for neurotrophin-3 (NT-3). This is a tyrosine-protein kinase receptor. Known substrates for the trk receptors are SHC1, PI-3 kinase, and PLCG1. The different isoforms do not have identical signaling properties.
- 组织特异性Widely expressed but mainly in nervous tissue. Isoform B is expressed at higher levels in adult brain than in fetal brain.
- 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 protein kinase domain. - 翻译后修饰Ligand-mediated auto-phosphorylation.
- 细胞定位Membrane.
- Information by UniProt
Recombinant Human TrkC protein (Fc Chimera) 图像
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SDS-PAGE - TrkC – Fc Chimera (ab85599)1D SDS-PAGE of ab85599 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab85599
Lane 2: ab85599 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab85599 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
5 mg of protein was loaded per lane. Gel was stained with Deep Purple™.Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Subsequent tightening of bands after treatment with glycosidase cocktail suggests the presence of O-linked glycans.
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Functional Studies - TrkC – Fc Chimera (ab85599)Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified Human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
The triangle indicates the theoretical MW and pI of the protein.
Recombinant Human TrkC protein (Fc Chimera) (ab85599)参考文献
ab85599 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"