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Our Abpromise guarantee covers the use of ab123775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Kd = 1.95 nM. Assay conditions: 20 mM HEPES, pH 7.5, 50 mM KCl, 5 mM MgCl2, 20 mM sodium molybdate, 2 mM DTT, 0.01% NP40, 100 μg/ml BSA, 50 nM TRAP1, geldanamycin-FITC (titration range 0.016-16 nM) incubated at 4oC for 24 hrs. Fluorescence polarization measured at RT (Ex/Em= 485/520 nm)
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 1.06% Potassium phosphate, 0.008% DTT, 0.015% EDTA, 2.5% Glycerol, 0.29% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ab123775 has not yet been referenced specifically in any publications.