Recombinant人TRAF6 protein (ab112366)

概述

描述

  • 性质Recombinant
  • 来源Wheat germ
  • 氨基酸序列
    • AccessionQ9Y4K3
    • 种属Human
    • 序列TMQGEYDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPELLAFQRP TIPRNPKGFGYVTFMHLEALRQRTFIKDDTLLVRCEVSTRFDMGSLRREG FQPRSTDAGV
    • 分子量38 kDa including tags
    • 氨基酸413 to 522

技术指标

Our Abpromise guarantee covers the use of ab112366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性useful for Antibody Production and Protein Array
  • 应用

    SDS-PAGE

    Western blot

    ELISA

  • 形式Liquid
  • 补充说明ab112366 is best used within three months from the date of receipt.useful for Antibody Production and Protein Array
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.79% Tris HCl, 0.31% Glutathione
    Note: Glutathione is reduced.

常规信息

  • 别名
    • E3 ubiquitin-protein ligase TRAF6
    • Interleukin 1 signal transducer
    • Interleukin-1 signal transducer
    • MGC 3310
    • MGC:3310
    • MGC3310
    • OTTHUMP00000232772
    • OTTHUMP00000232773
    • RING finger protein 85
    • RNF 85
    • RNF85
    • TNF receptor associated factor 6
    • TNF receptor-associated factor 6
    • TNF receptor-associated factor 6, E3 ubiquitin protein ligase
    • TRAF 6
    • Traf6
    • TRAF6_HUMAN
    see all
  • 功能E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor.
  • 组织特异性Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the TNF receptor-associated factor family. A subfamily.
    Contains 1 MATH domain.
    Contains 1 RING-type zinc finger.
    Contains 2 TRAF-type zinc fingers.
  • 结构域The coiled coil domain mediates homo- and hetero-oligomerization.
    The MATH/TRAF domain binds to receptor cytoplasmic domains.
  • 翻译后修饰Sumoylated on Lys-124, Lys-142 and Lys-453 by SUMO1.
    Polyubiquitinated on Lys-124; after cell stimulation with IL-1-beta or TGF-beta. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form.
  • 细胞定位Cytoplasm. Cytoplasm > cell cortex. Nucleus. Found in the nuclei of some agressive B-cell lymphoma cell lines as well as in the nuclei of both resting and activated T-and B-lymphocytes. Found in punctate nuclear body protein complexes. Ubiquitination may occur in the cytoplasm and sumoylation in the nucleus.
  • Information by UniProt

Recombinant Human TRAF6 protein 图像

  • ab112366 analysed by 12.5% SDS-PAGE and stained with Coomassie Blue.

Recombinant Human TRAF6 protein (ab112366)参考文献

ab112366 has not yet been referenced specifically in any publications.

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