Recombinant人TPA组织Plasminogen Activator protein (FITC) (ab92679)



  • 性质Recombinant
  • 来源CHO cells
  • 氨基酸序列
    • 种属Human
  • 偶联物FITC. Ex: 493nm, Em: 528nm


Our Abpromise guarantee covers the use of ab92679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性It can form 100% complex formation with PAI1.
  • 应用


  • 纯度> 95 % SDS-PAGE.
    >85 percent single chain.
  • 形式Liquid
  • 补充说明Protect from light.It can form 100% complex formation with PAI1.
  • Concentration information loading...


  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 0.4M HEPES, 0.1M Sodium chloride, pH 7.4


  • 别名
    • Alteplase
    • DKFZp686I03148
    • Plasminogen activator tissue
    • Plasminogen activator tissue type
    • PLAT
    • Reteplase
    • t PA
    • T Plasminogen Activator
    • t-PA
    • T-plasminogen activator
    • Tissue plasminogen activator (t PA)
    • Tissue type plasminogen activator
    • Tissue-type plasminogen activator chain B
    • tPA
    • TPA1
    see all
  • 功能Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.
  • 组织特异性Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
  • 疾病相关Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 fibronectin type-I domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • 结构域Both FN1 and one of the kringle domains are required for binding to fibrin.
    Both FN1 and EGF-like domains are important for binding to LRP1.
    The FN1 domain mediates binding to annexin A2.
    The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.
  • 翻译后修饰The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
    Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
    N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
    Characterization of O-linked glycan was studied in Bowes melanoma cell line.
  • 细胞定位Secreted > extracellular space.
  • Information by UniProt

Recombinant Human TPA Tissue Plasminogen Activator protein (FITC) 图像

  • Staining of TPA Tissue Plasminogen Activator protein by 10% SDS-PAGE (UV trans-illuminator).
    Lane 1: ab92679 (3 µg) reduced
    Lane 2: ab92679 (3 µg) non-reduced
  • Staining of TPA Tissue Plasminogen Activator protein by 10% SDS-PAGE.
    Lane 1: ab92679 (3 µg) reduced
    Lane 2: ab92679 (3 µg) non-reduced
    Lane 3: Prestained standard

Recombinant Human TPA Tissue Plasminogen Activator protein (FITC) (ab92679)参考文献

ab92679 has not yet been referenced specifically in any publications.

Product Wall

Application Other

Review text: Prior to using for fluoresecent confocal microscopy ab92679 (FITC-tPA) was tested for for fibrinolytic activity by measuring clot lysis using a turbidity assay. Clot lysis was observed with 20 pM ab92679.
For observation of lysis by fluorescent confocal microscopy 75 nM ab92679 was used. A signal could be detected as expected at the lysis front.
Sample: Human Recombinant protein

Primary antibody (in addition to 'TPA Tissue Plasminogen Activator protein (FITC)')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used

Dr. Claire Whyte

Verified customer

提交于 Nov 27 2012

Thank you for sending the images.
They are now added and I also would like to confirm that the discount codes are active and ready to be used for a further purchase.
The reviews will be allocated to one of team soon and then will soon be publ...

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Thank you for your email Please excuse the late response, Cathrin is not in the office at the moment.
I am happy to confirm that I added the discount codes to the internal notes in the reviews and therefore the codes are no activated.
If you ...

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I am very pleased to hear you would like to accept our offer and test ab92679 and ab93152 in Functional Studies. This code will give you: 1 free product per code before the expiration date. To redeem this offer, please submit an Abreview for Funct...

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Thank you very much for your interest in our products.
As we haven't tested this ourself, it is hard to predict if these proteins will work in a functional study. Therefore, I can offer a discount off a future purchase if you buy ab92679 and ab931...

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