Recombinant人TPA组织Plasminogen Activator protein (ab92637)

概述

描述

  • 性质
    Recombinant
  • 来源
    CHO cells
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 氨基酸
      36 to 562

技术指标

Our Abpromise guarantee covers the use of ab92637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Specific activity: 3,147,288 IU/mg (Relative to WHO International Standard for Human Recombinant Tissue Plasminogen Activator, NIBSC 98/714).
  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.
    Activated from single-chain form with immobilized plasmin. 100 percent complex formation with Human PAI1.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 0.4M HEPES, 0.1M Sodium chloride, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Alteplase
    • DKFZp686I03148
    • Plasminogen activator tissue
    • Plasminogen activator tissue type
    • PLAT
    • Reteplase
    • t PA
    • T Plasminogen Activator
    • t-PA
    • T-plasminogen activator
    • Tissue plasminogen activator (t PA)
    • Tissue type plasminogen activator
    • Tissue-type plasminogen activator chain B
    • tPA
    • TPA_HUMAN
    • TPA1
    see all
  • 功能
    Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.
  • 组织特异性
    Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.
  • 疾病相关
    Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.
  • 序列相似性
    Belongs to the peptidase S1 family.
    Contains 1 EGF-like domain.
    Contains 1 fibronectin type-I domain.
    Contains 2 kringle domains.
    Contains 1 peptidase S1 domain.
  • 结构域
    Both FN1 and one of the kringle domains are required for binding to fibrin.
    Both FN1 and EGF-like domains are important for binding to LRP1.
    The FN1 domain mediates binding to annexin A2.
    The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.
  • 翻译后修饰
    The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
    Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
    N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
    Characterization of O-linked glycan was studied in Bowes melanoma cell line.
  • 细胞定位
    Secreted > extracellular space.
  • Information by UniProt

图片

  • 10% SDS-PAGE
    Lane 1: TPA Tissue Plasminogen Activator (3 ug) Non-reduced
    Lane 2: TPA Tissue Plasminogen Activator (3 ug) Reduced
    Lane 3: Prestained Standard

文献

This product has been referenced in:
  • Rossmeisl JH  et al. Expression and activity of the urokinase plasminogen activator system in canine primary brain tumors. Onco Targets Ther 10:2077-2085 (2017). Read more (PubMed: 28442916) »

See 1 Publication for this product

客户评价及客户问答

This protein is 70 kDa.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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