重组人组织Plasminogen活化剂蛋白(Active) (ab92637)
Key features and details
- Expression system: CHO cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人组织Plasminogen活化剂蛋白(Active)
参阅全部 Tissue Plasminogen Activator 蛋白酶 -
生物活性
3,147,288 IU/mg (Relative to WHO International Standard for Human Recombinant Tissue Plasminogen Activator, NIBSC 98/714).
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纯度
> 95 % SDS-PAGE.
Activated from single-chain form with immobilized plasmin. 100% complex formation with Human PAI1. -
表达系统
CHO cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
SYQVICRDEK TQMIYQQHQS WLRPVLRSNR VEYCWCNSGR AQCHSVPVKS CSEPRCFNGG TCQQALYFSD FVCQCPEGFA GKCCEIDTRA TCYEDQGISY RGTWSTAESG AECTNWNSSA LAQKPYSGRR PDAIRLGLGN HNYCRNPDRD SKPWCYVFKA GKYSSEFCST PACSEGNSDC YFGNGSAYRG THSLTESGAS CLPWNSMILI GKVYTAQNPS AQALGLGKHN YCRNPDGDAK PWCHVLKNRR LTWEYCDVPS CSTCGLRQYS QPQFRIKGGL FADIASHPWQ AAIFAKHRRS PGERFLCGGI LISSCWILSA AHCFQERFPP HHLTVILGRT YRVVPGEEEQ KFEVEKYIVH KEFDDDTYDN DIALLQLKSD SSRCAQESSV VRTVCLPPAD LQLPDWTECE LSGYGKHEAL SPFYSERLKE AHVRLYPSSR CTSQHLLNRT VTDNMLCAGD TRSGGPQANL HDACQGDSGG PLVCLNDGRM TLVGIISWGL GCGQKDVPGV YTKVTNYLDW IRDNMRP -
预测分子量
59 kDa -
氨基酸
36 to 562 -
额外的序列信息
Full length mature protein, without the propeptides and signal peptide.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab92637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
Extinction Coefficient: 1.9
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.40
Constituents: 9.52% HEPES, 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Alteplase
- DKFZp686I03148
- Plasminogen activator tissue
see all -
功能
Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration. -
组织特异性
Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk. -
疾病相关
Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. -
序列相似性
Belongs to the peptidase S1 family.
Contains 1 EGF-like domain.
Contains 1 fibronectin type-I domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
结构域
Both FN1 and one of the kringle domains are required for binding to fibrin.
Both FN1 and EGF-like domains are important for binding to LRP1.
The FN1 domain mediates binding to annexin A2.
The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site. -
翻译后修饰
The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
Characterization of O-linked glycan was studied in Bowes melanoma cell line. -
细胞定位
Secreted > extracellular space. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (5)
ab92637 被引用在 5 文献中.
- Baumann J et al. Reduced platelet forces underlie impaired hemostasis in mouse models of MYH9-related disease. Sci Adv 8:eabn2627 (2022). PubMed: 35584211
- Bosch-Sanz O et al. 1,2,3-Triazole Derivatives as Novel Antifibrinolytic Drugs. Int J Mol Sci 23:N/A (2022). PubMed: 36499270
- Auderset L et al. Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Is a Negative Regulator of Oligodendrocyte Progenitor Cell Differentiation in the Adult Mouse Brain. Front Cell Dev Biol 8:564351 (2020). PubMed: 33282858
- Rossmeisl JH et al. Expression and activity of the urokinase plasminogen activator system in canine primary brain tumors. Onco Targets Ther 10:2077-2085 (2017). PubMed: 28442916
- Li QK et al. Serum fucosylated prostate-specific antigen (PSA) improves the differentiation of aggressive from non-aggressive prostate cancers. Theranostics 5:267-76 (2015). PubMed: 25553114