Recombinant人TNIK protein (ab119725)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • AccessionQ9UKE5
    • 种属Human
    • 分子量67 kDa including tags
    • 氨基酸1 to 367
    • 标签GST tag N-Terminus

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技术指标

Our Abpromise guarantee covers the use of ab119725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性ab119725 specific activity determined to be 68 nmol/min/mg.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    The purity was determined to be 95% by densitometry
  • 形式Liquid
  • 补充说明

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.79% Tris HCl, 0.88% Sodium chloride, 25% Glycerol, 0.31% Glutathione, 0.003% EDTA, 0.002% PMSF, 0.003% DTT

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • EC 2.7.11.1
    • KIAA0551
    • Tnik
    • TNIK_HUMAN
    • Traf2 and NCK interacting kinase
    • Traf2 and NCK-interacting protein kinase
    see all
  • 功能Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading.
  • 组织特异性Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CNH domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated. Autophosphorylation is activated by RAP2A and induces association to the cytoskeletal fraction.
  • 细胞定位Nucleus. Cytoplasm. Recycling endosome. Cytoplasm > cytoskeleton. Associated with recycling endosomes and the cytoskeletal fraction upon RAP2A overexpression.
  • Information by UniProt

Recombinant human TNIK protein 图像

  • SDS Page analysis of ab119725
  • ab119725 specific activity determined to be 68 nmol/min/mg.

Recombinant human TNIK protein (ab119725)参考文献

ab119725 has not yet been referenced specifically in any publications.

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