Recombinant人TNF Receptor I protein (Fc Chimera) (ab83577)

概述

描述

  • 性质Recombinant
  • 来源HEK 293 cells
  • 氨基酸序列
    • AccessionP19438
    • 种属Human
    • 序列Theoretical Sequence: IYPSGVIGLVPHLGDREKRDSVCPQGKYIHPQNNSICCT KCHKGTYLY NDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKE MGQVEISSC TVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHL SCQEKQNTV CTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIENVKG TEDSGIPKV DKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDT LMISRTPEV TCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNS TYRVVSVLT VLHQDWLNGKEYKCRVSNKALPAPIEKTISKAKGQPREP QVYTLPPSR DELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTT PPVLDSDGS FFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLS LSPGK
    • 氨基酸22 to 209
    • 额外的序列信息Fusion of aa 1-209 of human TNF Receptor 1 and aa 93-330 of Fc region of human IgG1 (P01857). The chimeric protein was expressed in modified human 293 cells.

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技术指标

Our Abpromise guarantee covers the use of ab83577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Activity: The ND50 of ab83577 is typically 15-30 ng/ml as measured by its ability to neutralize TNF-a mediated cytoxicity in murine WEHI 164 cells in the presence of actinomycin D.
  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

常规信息

  • 别名
    • CD120a
    • FPF
    • MGC19588
    • p55
    • p55-R
    • p60
    • TBP1
    • TBPI
    • TNF R
    • TNF R55
    • TNF-R1
    • TNF-RI
    • TNFAR
    • TNFR-I
    • TNFR1
    • TNFR55
    • TNFR60
    • TNFRI
    • TNFRSF1a
    • TNR1A_HUMAN
    • Tumor necrosis factor receptor 1
    • Tumor necrosis factor receptor superfamily, member 1A
    • Tumor necrosis factor receptor type 1
    • Tumor necrosis factor receptor type I
    • Tumor necrosis factor-binding protein 1
    see all
  • 功能Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.
  • 疾病相关Familial hibernian fever
    Multiple sclerosis 5
  • 序列相似性Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • 结构域The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.
    Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP.
  • 翻译后修饰The soluble form is produced from the membrane form by proteolytic processing.
  • 细胞定位Cell membrane. Golgi apparatus membrane. Secreted. A secreted form is produced through proteolytic processing and Secreted. Lacks a Golgi-retention motif, is not membrane bound and therefore is secreted.
  • Information by UniProt

Recombinant human TNF Receptor I protein (Fc Chimera) 图像

  • Densitometry scan demonstrating the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification. The triangle indicates the theoretical MW and pI of the protein.
  • 1D SDS-PAGE of ab83577 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1 – MW markers; Lane 2 – ab83577 ; Lane 3 – ab83577 treated with PNGase F to remove potential N-linked glycans; Lane 4 – ab83577 treated with a glycosidase cocktail to remove potential N- and O-linked glycans. 10 µg protein loaded per lane.

    Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Slight drop in MW after treatment with glycosidase cocktail suggests presence of O-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes.

Recombinant human TNF Receptor I protein (Fc Chimera) (ab83577)参考文献

ab83577 has not yet been referenced specifically in any publications.

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