Recombinant人TNF alpha protein (ab9642)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP01375
    • 种属Human
    • 序列VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL
    • 分子量17 kDa
    • 氨基酸77 to 233

技术指标

Our Abpromise guarantee covers the use of ab9642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D is ≤ 0.05 ng/mL, corresponding to a specific activity of ≥ 2 x 107 units/mg.

  • 应用

    Functional Studies

    Sandwich ELISA

    SDS-PAGE

  • 内毒素水平< 0.100 Eu/µg
  • 纯度> 98 % SDS-PAGE.
    Sterile filtered. Greater than 97% by SDS-PAGE and HPLC analyses.
  • 形式Lyophilised
  • 补充说明

    Lots prior to June 2015 contain 0.036% Tris (including lots GR157466-11, -12 and -13).

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Constituents: 0.049% Sodium phosphate, 0.12% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstitute in 500ul dH2O.

常规信息

  • 别名
    • APC1
    • APC1 protein
    • Cachectin
    • DIF
    • Differentiation inducing factor
    • Macrophage cytotoxic factor
    • Tnf
    • TNF superfamily, member 2
    • TNF, macrophage derived
    • TNF, monocyte derived
    • TNF-a
    • TNF-alpha
    • TNFA
    • TNFA_HUMAN
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    • Tumor Necrosis Factor, Membrane Form
    • Tumor necrosis factor, soluble form
    see all
  • 功能Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 疾病相关Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 序列相似性Belongs to the tumor necrosis factor family.
  • 翻译后修饰The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt

Recombinant human TNF alpha protein 图像

Recombinant human TNF alpha protein (ab9642)参考文献

This product has been referenced in:
  • Gasparotto J  et al. Effects of different products of peach (Prunus persica L. Batsch) from a variety developed in southern Brazil on oxidative stress and inflammatory parameters in vitro and ex vivo. J Clin Biochem Nutr 55:110-9 (2014). Read more (PubMed: 25320458) »
  • Paludo FJ  et al. Effects of 47C allele (rs4880) of the SOD2 gene in the production of intracellular reactive species in peripheral blood mononuclear cells with and without lipopolysaccharides induction. Free Radic Res 48:190-9 (2014). Read more (PubMed: 24164232) »

See all 3 Publications for this product

Product Wall


The lab let me know that they do not test the half-life/stability of ab9642 at 37oC. However, they have found that in their assay on 929 cells, they incubate this product in culture for approximately 21 hours. It is incubated in assay media cont...

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Thank you for your enquiry. As I mentioned earlier, these proteins were originated from two different sources outside Abcam. Regarding the specific activity and purity for these antibodies: Ab7420 - Specific activity: 2.34 x 10 to the 6 Units/mg prote...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"