Recombinant人TIMP2 protein (ab82117)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP16035
    • 种属Human
    • 序列CSCSPVHPQ QAFCNADVVI RAKAVSEKEV DSGNDIYGNP IKRIQYEIKQ IKMFKGPEKD IEFIYTAPSS AVCGVSLDVG GKKEYLIAGK AEGDGKMHIT LCDFIVPWDT LSTTQKKSLN HRYQMGCECK ITRCPMIPCY ISSPDECLWM DWVTEKNING HQAKFFACIK RSDGSCAWYR GAAPPKQEFL DIEDP
    • 氨基酸2 to 220

技术指标

Our Abpromise guarantee covers the use of ab82117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Biological Activity : TIMP2 activity was measured by its ability to inhibit human MMP1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP2 concentration of approximately 0.5 µg/ml, when using an MMP1 concentration of 1.6 µg/ml.
  • 应用

    SDS-PAGE

    Functional Studies

  • 内毒素水平< 0.100 Eu/µg
  • 纯度> 95 % SDS-PAGE.
    Purity is greater than 95% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • CSC 21K
    • CSC-21K
    • CSC21K
    • Metalloproteinase inhibitor 2
    • Metalloproteinase inhibitor 2 precursor
    • TIMP 2
    • TIMP metallopeptidase inhibitor 2
    • TIMP-2
    • TIMP2
    • TIMP2_HUMAN
    • Tissue Inhibitor of Metalloproteinase 2
    • Tissue inhibitor of metalloproteinases 2
    see all
  • 功能Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
  • 序列相似性Belongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • 翻译后修饰The activity of TIMP2 is dependent on the presence of disulfide bonds.
  • 细胞定位Secreted.
  • Information by UniProt

Recombinant human TIMP2 protein (ab82117)参考文献

This product has been referenced in:
  • Dohi T  et al. Tissue Inhibitor of Metalloproteinase-2 Suppresses Collagen Synthesis in Cultured Keloid Fibroblasts. Plast Reconstr Surg Glob Open 3:e520 (2015). ELISA . Read more (PubMed: 26495233) »

See 1 Publication for this product

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Please find the reconstitution instructions for your lot of ab82117 below:

Reconstitution:

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do n...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"