Recombinant人Thymine DNA glycosylase protein (ab114876)

概述

描述

  • 性质Recombinant
  • 来源Wheat germ
  • 氨基酸序列
    • AccessionQ13569
    • 种属Human
    • 序列MEAENAGSYSLQQAQAFYTFPFQQLMAEAPNMAVVNEQQMPEEVPAPAPA QEPVQEAPKGRKRKPRTTEPKQPVEPKKPVESKKSGKSAKSKEKQEKITD TFKVKRKVDRFNGVSEAELLTKTLPDILTFNLDIVIIGINPGLMAAYKGH HYPGPGNHFWKCLFMSGLSEVQLNHMDDHTLPGKYGIGFTNMVERTTPGS KDLSSKEFREGGRILVQKLQKYQPRIAVFNGKCIYEIFSKEVFGVKVKNL EFGLQPHKIPDTETLCYGMPSSSARCAQFPRAQDKVHYYIKLKDLRDQLK GIERNMDVQEVQYTFDLQLAQEDAKKMAVKEEKYDPGYEAAYGGAYGENP CSSEPCGFSSNGLIESVELRGESAFSGIPNGQWMTQSFTDQIPSFSNHCG TQEQEEESHA
    • 分子量71 kDa including tags
    • 氨基酸1 to 410
    • 标签GST tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab114876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    SDS-PAGE

    Western blot

  • 形式Liquid
  • 补充说明

    Best use within three months from the date of receipt of this protein.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

常规信息

  • 别名
    • C JUN leucine zipper interactive protein
    • C-JUN leucine zipper interactive protein JZA-3
    • E130317C12Rik
    • EC 3.2.2.29
    • G/T mismatch specific thymine DNA glycosylase
    • G/T mismatch-specific thymine DNA glycosylase
    • JZA 3
    • Jza1
    • T:G mismatch thymine glycosylase
    • Tdg
    • TDG_HUMAN
    • Thymine DNA glycosylase
    • Thymine-DNA glycosylase
    see all
  • 功能In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.
  • 序列相似性Belongs to the TDG/mug DNA glycosylase family.
  • 翻译后修饰Sumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA.
  • 细胞定位Nucleus.
  • Information by UniProt

Recombinant Human Thymine DNA glycosylase protein 图像

  • 12.5% SDS-PAGE showing ab114876 at approximately 71.17kDa.
    Stained with Coomassie Blue.

Recombinant Human Thymine DNA glycosylase protein (ab114876)参考文献

ab114876 has not yet been referenced specifically in any publications.

Product Wall

I can confirm that this product (ab114876: Human Thymine DNA glycosylase full length protein) has been tested and characterised for the use of ELISA, SDS-PAGE and Western blot. We have not done any tests for enzymatic activity yet, so we are not sure i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"