Recombinant人Thioredoxin 2 protein (ab78845)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MTTFNIQDGP DFQDRVVNSE TPVVVDFHAQ WCGPCKILGP RLEKMVAKQH GKVVMAKVDI DDHTDLAIEY EVSAVPTVLA MKNGDVVDKF VGIKDEDQLE AFLKKLIG

技术指标

Our Abpromise guarantee covers the use of ab78845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity is 3-4 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    ab78845 is purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • mitochondrial thioredoxin
    • MT-TRX
    • MTRX
    • thioredoxin 2 precursor
    • Thioredoxin mitochondrial
    • Thioredoxin2
    • TRX 2
    • TRX2
    • TXN 2
    • TXN2
    see all
  • 相关性Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide isomerase(PDI) and a number of transcription factors such as p53, NF-kB and AP-1. Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.
  • 细胞定位Mitochondrial

Recombinant human Thioredoxin 2 protein 图像

  • 15% SDS-PAGE showing ab78845 at approximately 11kDa (3µg).

Recombinant human Thioredoxin 2 protein (ab78845)参考文献

ab78845 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your call yesterday and for your questions about ab78845.
I've heard back from the lab, and I apologize but I was incorrect. We haven’t tested it for biological activity on cells at this time. We have tested it in an insulin pr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"