重组人TGF beta 1蛋白(ab50038)

Submit an Abreview Q&A (3)

概述

描述

  • 性质
    Recombinant
  • 来源
    CHO cells
  • 氨基酸序列
    • 种属
      Human
    • 序列
      ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
    • 分子量
      25 kDa
    • 额外的序列信息
      ab50038 composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond.

技术指标

Our Abpromise guarantee covers the use of ab50038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    The ED50 was determined by TGF beta 1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is < 0.05 ng/ml, corresponding to a specific activity of >2 x 107 units/mg

  • 应用

    SDS-PAGE

    Functional Studies

  • 形式
    Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶
    Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

常规信息

  • 别名
    • Cartilage-inducing factor
    • CED
    • Differentiation inhibiting factor
    • DPD1
    • LAP
    • Latency-associated peptide
    • Prepro transforming growth factor beta 1
    • TGF beta
    • TGF beta 1
    • TGF beta 1 protein
    • TGF-beta 1 protein
    • TGF-beta-1
    • TGF-beta-5
    • TGF-beta1
    • TGFB
    • Tgfb-1
    • tgfb1
    • TGFB1_HUMAN
    • TGFbeta
    • TGFbeta1
    • Transforming Growth Factor b1
    • Transforming Growth Factor beta 1
    • Transforming growth factor beta 1a
    • transforming growth factor beta-1
    • transforming growth factor, beta 1
    • Transforming Growth Factor-ß1
    see all
  • 功能
    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • 组织特异性
    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • 疾病相关
    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • 序列相似性
    Belongs to the TGF-beta family.
  • 翻译后修饰
    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

文献

ab50038 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab50038？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

提交评价 提交问题

3 reviews or Q&A

10
Votes: Highest First

I would like touse human recombinant TGF beta1 proteinformouse cells. I wonder which one is the best for me?
Code# Ab50036 seems good for my purpose.What does it mean ACTIVE for others (ab50038, ab116150)?

Thank you so much!

Thank you for contacting us.

I assume you would like to use the protein for functional studies? From the looks of it, all three of them seem to be suitable for Functional Studies, but withinAb50036the endotoxin level has been determined as w...

Read More

I would like to know if ab50038 is secreted and then purified from growth medium or purified from lysed cells.

The TGF-beta 1 is secreted from the CHO cells, the media is then collected and the protein is isolated and purified. Please do not hesitate to contact me should you have any additional questions.

Inquiry: Hello, I am looking for a preparation of TGFB-1 to use for in vitro induction of EMT in human cell lines. Which of your products would you recommend to this kind of assay? Best regards,

Thank you for your enquiry.

I have reviewed with our sources the TGF beta 1 proteins that we have on our catalog. It seems the most suitable would be ab50036 TGF beta 1 protein (Active).

http://www.abcam.com/index.html?datasheet=5...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册