重组人TATA binding蛋白TBP (ab81897)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: GSA, SDS-PAGE, WB
描述
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产品名称
重组人TATA binding蛋白TBP
参阅全部 TATA binding protein TBP 蛋白酶 -
生物活性
1 unit equals 1 nanogram of purified protein. -
纯度
> 95 % SDS-PAGE. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MDQNNSLPPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQ NTNSL SILEEQQRQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQAVAA AAV QQSTSQ QATQGTSGQA PQLFHSQTLT TAPLPGTTPL YPSPMTPMTP ITPATPASES SG IVPQLQN IVSTVNLGCK LDLKTIALRA RNAEYNPKRF AAVIMRIREP RTTALIFSSG K MVCTGAKS EEQSRLAARK YARVVQKLGF PAKFLDFKIQ NMVGSCDVKF PIRLEGLVLT HQQFSSYEP ELFPGLIYRM IKPRIVLLIF VSGKVVLTGA KVRAEIYEAF ENIYPILKG FRKTT -
预测分子量
39 kDa -
氨基酸
1 to 339 -
标签
His tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab81897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Gel Supershift Assays
SDS-PAGE
Western blot
EMSA
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形式
Liquid -
补充说明
1 unit equals 1 nanogram of purified protein. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.9
Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)
常规信息
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别名
- GTF2D
- GTF2D1
- HDL4
see all -
功能
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. -
组织特异性
Widely expressed, with levels highest in the testis and ovary. -
疾病相关
Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. -
序列相似性
Belongs to the TBP family. -
细胞定位
Nucleus. - Information by UniProt
图片
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All lanes : Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade (ab63766) at 1 µg/ml
Lane 1 :Recombinant Human TATA binding protein TBP (ab81897) at 0.1 µg
Lane 2 :Recombinant Human TATA binding protein TBP (ab81897) at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab81897 被引用在 1 文献中.
- Grabherr MG et al. Exploiting nucleotide composition to engineer promoters. PLoS One 6:e20136 (2011). PubMed: 21625601