Recombinant人Survivin protein (ab87202)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAKG SHMGAPTLPP AWQPFLKDHR ISTFKNWPFL EGCACTPERM AEAGFIHCPT ENEPDLAQCF FCFKELEGWE PDDDPIEEHK KHSSGCAFLS VKKQFEELTL GEFLKLDRER AKNKIAKETN NKKKEFEETA KKVRRAIEQL AAMD

技术指标

Our Abpromise guarantee covers the use of ab87202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度> 90 % SDS-PAGE.
    ab87202 is purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 0.1M Sodium chloride, 20mM Tris, pH 7.5

常规信息

  • 别名
    • API4
    • Apoptosis inhibitor 4
    • Apoptosis inhibitor survivin
    • Apoptosis inhibitor4
    • Baculoviral IAP repeat containing 5
    • Baculoviral IAP repeat containing protein 5
    • Baculoviral IAP repeat-containing protein 5
    • BIRC 5
    • BIRC5
    • BIRC5_HUMAN
    • EPR 1
    • IAP4
    • Survivin variant 3 alpha
    • SVV
    • TIAP
    see all
  • 功能Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May play a role in neoplasia. May counteract a default induction of apoptosis in G2/M phase. Inhibitor of caspase-3 and caspase-7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
  • 组织特异性Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
  • 序列相似性Belongs to the IAP family.
    Contains 1 BIR repeat.
  • 发展阶段Expression is cell cycle-dependent and peaks at mitosis.
  • 结构域The BIR repeat is necessary and sufficient for HBXIP binding.
  • 翻译后修饰Ubiquitination is required for centrosomal targeting.
    In vitro phosphorylation at Thr-117 by AURKB/STK12 prevents interaction with INCENP and localization to mitotic chromosomes.
  • 细胞定位Cytoplasm. Nucleus. Chromosome. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle. Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with AURKB at mitotic chromosomes.
  • Information by UniProt

Recombinant Human Survivin protein 图像

Recombinant Human Survivin protein (ab87202)参考文献

This product has been referenced in:
  • Zeh HJ  et al. First-in-man study of western reserve strain oncolytic vaccinia virus: safety, systemic spread, and antitumor activity. Mol Ther 23:202-14 (2015). Read more (PubMed: 25292189) »

See 1 Publication for this product

Product Wall

The survivin protein ab87202 has a calmodulin tag (CaM) at the N terminus, which is about 17 kDa, as large as the survivn protein itself. You will still be able to biotinylate the survivin, just not at the N terminus, and the calmodulin protein will al...

Read More

Thank you for contacting Abcam regarding ab87202.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for the HeLa lysate ab29...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"