Recombinant人Src protein (ab51424)



  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 标签GST tag N-Terminus


Our Abpromise guarantee covers the use of ab51424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity 98 nmol/min/mg.
  • 应用

    Functional Studies


  • 形式Liquid
  • 补充说明

    ab204883 (CDK1 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...


  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.


  • 别名
    • ASV
    • Avian sarcoma virus
    • c SRC
    • CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA
    • cSrc
    • EC
    • Neuronal CSRC tyrosine specific protein kinase
    • Neuronal SRC
    • Oncogene SRC
    • OTTHUMP00000174476
    • OTTHUMP00000174477
    • p60 Src
    • p60-Src
    • p60Src
    • pp60c src
    • pp60c-src
    • pp60csrc
    • Proto oncogene tyrosine protein kinase Src
    • Proto-oncogene c-Src
    • Proto-oncogene tyrosine-protein kinase Src
    • Protooncogene SRC
    • Protooncogene SRC Rous sarcoma
    • Src
    • SRC Oncogene
    • SRC proto oncogene non receptor tyrosine kinase
    • SRC1
    • Tyrosine kinase pp60c src
    • Tyrosine protein kinase SRC 1
    • Tyrosine protein kinase SRC1
    • v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog
    • V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian)
    • v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian
    see all
  • 功能Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻译后修饰Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
    S-nitrosylation is important for activation of its kinase activity.
  • 细胞定位Cell membrane. Mitochondrion inner membrane.
  • Information by UniProt

Recombinant human Src protein 图像

  • Kinase activity assay - Specific activity 98 nmol/min/mg
  • Recombinant human Src protein (tagged). Molecular weight 83 kDa.

Recombinant human Src protein (ab51424)参考文献

ab51424 has not yet been referenced specifically in any publications.

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