Recombinant人SPHK2 protein (ab42625)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab42625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific Activity : 20 U/ug. One unit defined as the amount of enzyme that will transfer 1nmol phosphate to Tyr substrate per minute at pH 7.4 and 30deg.C. Assay buffer : 50mM HEPES pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3um Na-orthovanadate, 0.1M ATP, 30ug/ml Poly (Glu:Tyr) 4:1 substrate and 0.75ug/ml recombinant SPHK2.
  • 应用

    Inhibition Assay

  • 形式Liquid
  • 补充说明Source : Baculovirus infected Sf9 cells
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • SK 2
    • Sphingosine kinase 2
    • Sphingosine kinase type 2
    • SPHK2
    • SPHK2_HUMAN
    • SPK 2
    see all
  • 功能Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.
  • 序列相似性Contains 1 DAGKc domain.
  • 细胞定位Cytoplasm. Membrane and Lysosome membrane.
  • Information by UniProt

Recombinant human SPHK2 protein (ab42625)参考文献

ab42625 has not yet been referenced specifically in any publications.

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