重组人SIK2蛋白(ab89856)
Key features and details
- Expression system: Insect cells
- Purity: > 70% Densitometry
- Active: Yes
- Suitable for: SDS-PAGE, WB, Functional Studies
描述
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产品名称
重组人SIK2蛋白 -
生物活性
The specific activity of ab89856 was determined to be 112 nmol/min/mg.
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纯度
> 70 % Densitometry.
Affinity purified. -
表达系统
Insect cells -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human
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相关产品
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Related Products
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab89856 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Western blot
Functional Studies
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形式
Liquid -
补充说明
ab204852 (AMARA substrate peptide for SIK + AMPK) can be utilized as a substrate for assessing kinase activity
This product was previously labelled as Snf1lk2, Snf1lk+Snf1lk2+SIK3
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- DKFZp434K1115
- EC 2.7.11.1
- KIAA0781
see all -
功能
Phosphorylates 'Ser-794' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing TORCs, the CREB-specific coactivators. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
细胞定位
Cytoplasm. - Information by UniProt
图片
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The specific activity of SIK2 (ab89856) was determined to be 37 nmol/min/mg as per activity assay protocol and was equivalent to 128 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab89856
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SDS PAGE analysis of ab89856
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SDS-PAGE showing ab89856 at approximately 150kDa
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Kinase Assay demonstrating specific activity of ab89856
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab89856 尚未被引用在任何文献中。