Recombinant人SNAP25 protein (ab51048)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVML DEQGEQLERIEEGMDQINKD MKEAEKNLTDLGKFCGLCVCPCNKLKSS DAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTND ARENEM DENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEAN QRATKMLGSG
    • 分子量
      23 kDa
    • 氨基酸
      1 to 206
    • 标签
      His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab51048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.
    Recombinant human SNAP25, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50mM Sodium chloride, 20mM Tris HCl, 2mM DTT, 1mM EDTA, pH 7.5

常规信息

  • 别名
    • bA416N4.2
    • Bdr
    • CMS18
    • dJ1068F16.2
    • FLJ23079
    • HGNC:11132
    • MGC105414
    • MGC139754
    • Resistance to inhibitors of cholinesterase 4 homolog
    • RIC 4
    • RIC4
    • SEC 9
    • SEC9
    • SNAP
    • SNAP 25
    • SNAP-25
    • SNAP-25B
    • SNAP25
    • SNP 25
    • SNP25
    • SNP25_HUMAN
    • sp
    • SUP
    • Super protein
    • Synaptosomal associated 25 kDa protein
    • Synaptosomal associated protein
    • Synaptosomal associated protein 25
    • Synaptosomal associated protein 25kDa
    • Synaptosomal-associated 25 kDa protein
    • Synaptosomal-associated protein 25
    • Synaptosomal-associated protein, 25-KD
    see all
  • 功能
    t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
  • 组织特异性
    Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
  • 序列相似性
    Belongs to the SNAP-25 family.
    Contains 2 t-SNARE coiled-coil homology domains.
  • 翻译后修饰
    Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
  • 细胞定位
    Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
  • Information by UniProt

图片

  • 15% SDS-PAGE. The amount of protein loaded is 3ug.

    Molecular Weight: 25.4 kDa

文献

ab51048 has not yet been referenced specifically in any publications.

客户评价及客户问答

I have been in contact with our lab and products team and indeed there is a duplication here which we are currently trying to fix.

I can confirm that ab74529 and ab155885 are identical and ab87335 and ab51048 are also identical.

The ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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