Recombinant人SIRT3 protein (Tagged) (ab54333)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 分子量
      59 kDa
    • 氨基酸
      102 to 399
    • 标签
      GST tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab54333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    Specific Activity: 6 U/ug. One Unit =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 uM BPS HDAC substrate , 200 uM NAD+, and 20 ng/ul Sirtuin 3. Incubation condition: 30 min at 30ºC.

  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度
    > 90 % SDS-PAGE.

  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 50mM Tris HCl, 20mM Glutathione, 138mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • hSIRT 3
    • hSIRT3
    • Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase
    • NAD dependent deacetylase sirtuin 3 mitochondrial
    • NAD-dependent protein deacetylase sirtuin-3, mitochondrial
    • Regulatory protein SIR2 homolog 3
    • Silent mating type information regulation 2 S.cerevisiae homolog 3
    • Sir 2 like 3
    • SIR 2 like protein 3
    • SIR 3
    • SIR2 L3
    • Sir2 like 3
    • SIR2 like protein 3
    • SIR2-like protein 3
    • SIR2L3
    • SIR3_HUMAN
    • SIRT 3
    • SIRT3
    • Sirtuin 3
    • Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae)
    • Sirtuin type 3
    • Sirtuin3
    see all
  • 功能
    NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
  • 组织特异性
    Widely expressed.
  • 序列相似性
    Belongs to the sirtuin family. Class I subfamily.
    Contains 1 deacetylase sirtuin-type domain.
  • 翻译后修饰
    Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.
  • 细胞定位
    Mitochondrion matrix.
  • Information by UniProt

Recombinant human SIRT3 protein (Tagged) (ab54333)参考文献

ab54333 has not yet been referenced specifically in any publications.

Product Wall

Cette protéine est une protéine humaine de Sirtuin 3 et consiste des acides aminés 102-399 avec un tag GST au N terminale.

Le poids moléculaire de cette protéine devrait donc être de 59 kDa.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册