重组人SIRT2蛋白(ab79948)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人SIRT2蛋白
参阅全部 SIRT2 蛋白酶 -
生物活性
Specific Activity: 1.4 U/µg. One U =1 pmol/min.
Activity: =1.0 pmol/min/µg. Assay conditions: 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate, 500 µM NAD+ and Sirtuin 2 (125 - 8000 ng). Incubation condition: 30 min at 37°C followed by treatment with 50 µl of SIRT developer for 15 min at RT. Fluorescence intensity is measured at ex360/em460.
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纯度
> 90 % SDS-PAGE. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MASSLGSQKERLLDELTLEGVARYMQSERCRRVICLVGAGISTSAGIPDF RSPSTGLYDNLEKYHLPYPEAIFEISYFKKHPEPFFALAKELYPGQFKPT ICHYFMRLLKDKGLLLRCYTQNIDTLERIAGLEQEDLVEAHGTFYTSHCV SASCRHEYPLSWMKEKIFSEVTPKCEDCQSLVKPDIVFFGESLPARFFSC MQSDFLKVDLLLVMGTSLQVQPFASLISKAPLSTPRLLINKEKAGQSDPF LGMIMGLGGGMDFDSKKAYRDVAWLGECDQGCLALAELLGWKKELEDLVR REHASIDAQSLEHHHHHH -
预测分子量
36 kDa including tags -
氨基酸
50 to 356 -
标签
His tag C-Terminus -
额外的序列信息
NP_036369 36 kDa including tag
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab79948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- FLJ35621
- FLJ37491
- NAD dependent deacetylase sirtuin 2
see all -
功能
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. -
组织特异性
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. -
序列相似性
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
发展阶段
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. -
翻译后修饰
Phosphorylated at the G2/M transition of the cell cycle. -
细胞定位
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab79948 尚未被引用在任何文献中。